calcTM: Melting Temperature (Tm) of an oligo probe

Description Usage Arguments Details Value Note References

Description

Calculate the Tm of a set of DNA nucleotides

Usage

1
calcTM(probes)

Arguments

probes

character vector of DNA nucleotide sequences

Details

Calculates the melting temperature of the given oligos. Any 'N's in the sequence cause a value of NA for that oligo.

Value

A numeric vector of the same length as probes

Note

Implemented as a Perl script, so requires write and execute permissions on the current directory. Creates a file 'MeltingTemp.pl'.

References

by Joseba Bikandi, http://biophp.org


robertdouglasmorrison/DuffyTools documentation built on Dec. 7, 2018, 8:02 a.m.