calculate_area: Calculate area under spectral curve

In ssokolen/fluoroscripts: Scripts for Importing and Processing Spectrofluorometry Data

Description

Calculates the area under an emission spectra within specified channels, excited by given laser wavelength. The output is a dataframe to facilitate use within dplyr's do() function.

Usage

calculate_area(wavelengths, excitations, emissions, lasers, channels,
  threshold = 0.01)

Arguments

wavelengths	A vector of wavelengths (nm).
excitations	A vector of excitation intensities.
emissions	A vector of emmision intensities.
lasers	Vector of lasers exciting given proteins e.g. c(488, 642).
channels	Vector of channels for which spectral area is calculated e.g. c('530/30', '670LP').
threshold	Fraction of total spectral area observed in a given channel that is considered to be practically 0.

Value

A data frame with two columns – channel and area.

ssokolen/fluoroscripts documentation built on May 30, 2019, 8:43 a.m.