matrix_to_spectra: Convert excitation/emission matrix to excitation/emission...

In ssokolen/fluoroscripts: Scripts for Importing and Processing Spectrofluorometry Data

Description

Converts intesity data from an excitation/emission profile. First, the wavelength of maximum peak intensity is identified at each excitation. The relative intensity at maximum emission forms the excitations curve, while the relative intensity at peak excitation forms the emission curve.

Usage

matrix_to_spectra(excitation, emission, intensity, wavelengths = NA,
  basis = seq(300, 800, length.out = 200))

Arguments

excitation	A vector of excitations.
emission	A vector of emissions.
intensity	A vector of intensities corresponding to each excitation/emission pair.
basis	A sequence of wavelength values (in nm) at which the excitation and emission curves will be evaluated.

Value

A data.frame with three columns – wavelength, excitation, and emission (corresponding to the excitation and emission curves).

ssokolen/fluoroscripts documentation built on May 30, 2019, 8:43 a.m.