Build.Cyto: Build.Cyto

In steveyu323/SEURATEXT:

Description

The four quadrant: x+/y+ = 1 x+/y- = 2 x-/y- = 3 x-/y+ = 4 The function will add a $cyto field into the current seurat object to specify the quadant a cell belongs to

Usage

Build.Cyto(seurat.ob, x, y, x.thresh = 0, y.thresh = 0)

Arguments

seurat.ob	a seurat object type that have a count a count table inside with proper column name as cells and rowname as the gene
x	The gene name of the first cell marker
y	The gene name of the second cell marker
x.thresh	threshold to deem positive for the first cell marker
y.thresh	threshold to deem positive for the second cell marker

Value

filtered seurat object with $cyto field added used for further

steveyu323/SEURATEXT documentation built on Nov. 5, 2019, 9:38 a.m.