relic | R Documentation |
REgression on Logarithm of Internal Control probes (RELIC) correct for dye bias on whole array by utilizing the intensity values of paired internal control probes that monitor the two color channels.
relic (mdat,at_red,cg_grn)
mdat |
An object of class |
at_red |
an intensity matrix for Illumina control probes "NORM_A" and "NORM_T" |
cg_grn |
an intensity matrix for Illumina control probes "NORM_C" and "NORM_G" |
The Illumina MethylationEPIC BeadChip contains 85 pairs of internal normalization control probes (name with prefix NORM_A, NORM_T, NORM_G or NORM_C), while its predecessor, Illumina HumanMethyl-ation450 BeadChip contains 93 pairs. RELIC first performs a regression on the logarithms of the intensity values of the normalization control probes to derive a quantitative relationship between red and green channels, and then uses the relationship to correct for dye-bias on intensity values for whole array.
An object of class methDataSet
or MethylSet
depends on
input class.
Zongli Xu and Liang Niu
Zongli Xu, Sabine A. S. Langie, Patrick De Boever, Jack A. Taylor and Liang Niu, RELIC: a novel dye-bias correction method for Illumina Methylation BeadChip, BMC Genomics. 2017
Package preprocessENmix
if (require(minfiData)) {
##background correction and dye bias correction
#rgDataSet as input
path <- file.path(find.package("minfiData"),"extdata")
rgSet <- readidat(path = path,recursive = TRUE)
mdat <- preprocessENmix(rgSet,bgParaEst="oob",nCores=6,dyeCorr ="RELIC")
#RGChannelSet as input
mdat=preprocessENmix(RGsetEx,bgParaEst="oob",nCores=6,dyeCorr ="RELIC")
##dye bias correction only
#methDataSet as input
path <- file.path(find.package("minfiData"),"extdata")
rgSet <- readidat(path = path,recursive = TRUE)
ctrls <- getCGinfo(rgSet,type="ctrl")
ctrls <- ctrls[ctrls$Address %in% rownames(rgSet),]
ctrl_r <- assays(rgSet)$Red[ctrls$Address,]
ctrl_g <- assays(rgSet)$Green[ctrls$Address,]
CG.controls <- ctrls$Type %in% c("NORM_C", "NORM_G")
AT.controls <- ctrls$Type %in% c("NORM_A", "NORM_T")
cg_grn=ctrl_g[CG.controls,]
at_red=ctrl_r[AT.controls,]
rownames(cg_grn) = ctrls$ExtendedType[CG.controls]
rownames(at_red) = ctrls$ExtendedType[AT.controls]
mdat=getmeth(rgSet)
mdat <- relic(mdat,at_red,cg_grn)
#MethylSet as input
ctrls <- getProbeInfo(RGsetEx,type="Control")
ctrls <- ctrls[ctrls$Address %in% featureNames(RGsetEx),]
ctrl_r <- getRed(RGsetEx)[ctrls$Address,]
ctrl_g <- getGreen(RGsetEx)[ctrls$Address,]
CG.controls <- ctrls$Type %in% c("NORM_C","NORM_G")
AT.controls <- ctrls$Type %in% c("NORM_A","NORM_T")
cg_grn <- ctrl_g[CG.controls,]
at_red <- ctrl_r[AT.controls,]
rownames(cg_grn) = ctrls$ExtendedType[CG.controls]
rownames(at_red) = ctrls$ExtendedType[AT.controls]
mdat <- preprocessRaw(RGsetEx)
mdat <- relic(mdat,at_red,cg_grn)
}
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