Description Usage Arguments Value Author(s) References See Also Examples
Counts unique loci in a module, maps the marker data of a module to the all available markers by creating a bit matrix for values above the given quantiles. Created bit matrix contains either TRUE (above quantiles) or FALSE (below or equals to quantiles) values as a resuls of these comparisons. It returns the results (marker mapping and bit matrix)
1 | ssea.prepare.counts(locdata, nloci, quantiles)
|
locdata |
marker data |
nloci |
number of elements in markers list |
quantiles |
quantile points for test statistic |
res |
a data list with the following components: locus2row: mapped marker information observed: bit matrix that involves TRUEs and FALSEs |
Ville-Petteri Makinen
Shu L, Zhao Y, Kurt Z, Byars SG, Tukiainen T, Kettunen J, Orozco LD, Pellegrini M, Lusis AJ, Ripatti S, Zhang B, Inouye M, Makinen V-P, Yang X. Mergeomics: multidimensional data integration to identify pathogenic perturbations to biological systems. BMC genomics. 2016;17(1):874.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 | job.msea <- list()
job.msea$label <- "hdlc"
job.msea$folder <- "Results"
job.msea$genfile <- system.file("extdata",
"genes.hdlc_040kb_ld70.human_eliminated.txt", package="Mergeomics")
job.msea$marfile <- system.file("extdata",
"marker.hdlc_040kb_ld70.human_eliminated.txt", package="Mergeomics")
job.msea$modfile <- system.file("extdata",
"modules.mousecoexpr.liver.human.txt", package="Mergeomics")
job.msea$inffile <- system.file("extdata",
"coexpr.info.txt", package="Mergeomics")
job.msea$nperm <- 100 ## default value is 20000
## ssea.start() process takes long time while merging the genes sharing high
## amounts of markers (e.g. loci). it is performed with full module list in
## the vignettes. Here, we used a very subset of the module list (1st 10 mods
## from the original module file) and we collected the corresponding genes
## and markers belonging to these modules:
moddata <- tool.read(job.msea$modfile)
gendata <- tool.read(job.msea$genfile)
mardata <- tool.read(job.msea$marfile)
mod.names <- unique(moddata$MODULE)[1:min(length(unique(moddata$MODULE)),
10)]
moddata <- moddata[which(!is.na(match(moddata$MODULE, mod.names))),]
gendata <- gendata[which(!is.na(match(gendata$GENE,
unique(moddata$GENE)))),]
mardata <- mardata[which(!is.na(match(mardata$MARKER,
unique(gendata$MARKER)))),]
## save this to a temporary file and set its path as new job.msea$modfile:
tool.save(moddata, "subsetof.coexpr.modules.txt")
tool.save(gendata, "subsetof.genfile.txt")
tool.save(mardata, "subsetof.marfile.txt")
job.msea$modfile <- "subsetof.coexpr.modules.txt"
job.msea$genfile <- "subsetof.genfile.txt"
job.msea$marfile <- "subsetof.marfile.txt"
## run ssea.start() and prepare for this small set: (due to the huge runtime)
job.msea <- ssea.start(job.msea)
## Remove extremely big or small modules:
st <- tool.aggregate(job.msea$moddata$MODULE)
mask <- which((st$lengths >= job.msea$mingenes) &
(st$lengths <= job.msea$maxgenes))
pos <- match(job.msea$moddata$MODULE, st$labels[mask])
job.msea$moddata <- job.msea$moddata[which(pos > 0),]
## Construct hierarchical representation for modules, genes, and markers:
ngens <- length(job.msea$genes)
nmods <- length(job.msea$modules)
db <- ssea.prepare.structure(job.msea$moddata, job.msea$gendata,
nmods, ngens)
## Determine test cutoffs:
if(is.null(job.msea$quantiles)) {
lengths <- db$modulelengths
mu <- median(lengths[which(lengths > 0)])
job.msea$quantiles <- seq(0.5, (1.0 - 1.0/mu), length.out=10)
}
## Calculate hit counts:
nloci <- length(job.msea$loci)
hits <- ssea.prepare.counts(job.msea$locdata, nloci, job.msea$quantiles)
db <- c(db, hits)
## Remove the temporary files used for the test:
file.remove("subsetof.coexpr.modules.txt")
file.remove("subsetof.genfile.txt")
file.remove("subsetof.marfile.txt")
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