erssa_edger: Run edgeR for computed sample combinations

In zshao1/ERSSA: Empirical RNA-seq Sample Size Analysis

Run edgeR for computed sample combinations

Description

erssa_edger function runs classic edgeR method to identify differentially expressed (DE) genes for each sample combination computed by comb_gen function. A gene is considered to be differentially expressed by defined FDR (Default=0.05) and logFC (Default=1) values. As an option, the function can also save the edgeR topTags tables as csv files to the drive.

Usage

erssa_edger(
  count_table.filtered = NULL,
  combinations = NULL,
  condition_table = NULL,
  control = NULL,
  cutoff_stat = 0.05,
  cutoff_Abs_logFC = 1,
  save_table = FALSE,
  path = "."
)

Arguments

count_table.filtered	Count table pre-filtered to remove non- to low- expressing genes. Can be the output of count_filter function.
combinations	List of combinations that is produced by comb_gen function.
condition_table	A condition table with two columns and each sample as a row. Column 1 contains sample names and Column 2 contains sample condition (e.g. Control, Treatment).
control	One of the condition names that will serve as control.
cutoff_stat	The cutoff in FDR for DE consideration. Genes with lower FDR pass the cutoff. Default = 0.05.
cutoff_Abs_logFC	The cutoff in abs(logFC) for differential expression consideration. Genes with higher abs(logFC) pass the cutoff. Default = 1.
save_table	Boolean. When set to TRUE, function will, in addition, save the generated edgeR TopTags table as csv files. The files are saved on the drive in the working directory in a new folder named "ERSSA_edgeR_table". Tables are saved separately by the replicate level. Default = FALSE.
path	Path to which the files will be saved. Default to current working directory.

Details

The main function calls edgeR functions to perform exact test for each computed combinations generated by comb_gen. In all tests, the pair-wise test sets the condition defined in the object "control" as the control condition.

In typical usage, after each test, the list of differentially expressed genes are filtered by FDR and log2FC values and only the filtered gene names are saved for further analysis. However, it is also possible to save all of the generated TopTags table to the drive for additional analysis that is outside the scope of this package.

Value

A list of list of vectors. Top list contains elements corresponding to replicate levels. Each child list contains elements corresponding to each combination at the respective replicate level. The child vectors contain differentially expressed gene names.

Author(s)

Zixuan Shao, [e-mail]

References

Robinson MD, McCarthy DJ, Smyth GK (2010). “edgeR: a Bioconductor package for differential expression analysis of digital gene expression data.” Bioinformatics, 26(1), 139-140.

Examples

# load example filtered count_table, condition_table and combinations
# generated by comb_gen function
# example dataset containing 1000 genes, 4 replicates and 5 comb. per rep.
# level
data(count_table.filtered.partial, package = "ERSSA")
data(combinations.partial, package = "ERSSA")
data(condition_table.partial, package = "ERSSA")

#run erssa_edger with heart condition as control
deg.partial = erssa_edger(count_table.filtered.partial, combinations.partial,
 condition_table.partial, control='heart')

zshao1/ERSSA documentation built on July 19, 2023, 9:20 p.m.