inst/examples/example_aucellResults_class.R

In AUCell: AUCell: Analysis of 'gene set' activity in single-cell RNA-seq data (e.g. identify cells with specific gene signatures)

# This example is run using a fake expression matrix.
# Therefore, the output will be meaningless.


############# Fake run of AUCell #############
set.seed(123)
exprMatrix <- matrix(data=sample(c(rep(0, 5000), sample(1:3, 5000, replace=TRUE))),
                     nrow=20, 
                     dimnames=list(paste("Gene", 1:20, sep=""), 
                                   paste("Cell", 1:500, sep="")))
dim(exprMatrix)

# Running AUCell
cells_rankings <- AUCell_buildRankings(exprMatrix)
fewGenes <- sample(rownames(exprMatrix), 10)
otherGenes <- sample(rownames(exprMatrix), 5)
geneSets <- list(geneSet1=fewGenes,
                 geneSet2=otherGenes)
cells_AUC <- AUCell_calcAUC(geneSets, cells_rankings, aucMaxRank=5, nCores=1)
##############################################

#Exploring the output:
cells_AUC

class(cells_AUC)

# Extracting the AUC matrix:
getAUC(cells_AUC)[,1:5]

# Subsetting and regular manipulation methods are also available:
cells_AUC[1:2,]
cells_AUC[,3:4]

dim(cells_AUC)
nrow(cells_AUC)
ncol(cells_AUC)
colnames(cells_AUC)
rownames(cells_AUC)

### Merging 2 objects (ranking or AUC):
sample1 <- cells_AUC[,10:20]
sample2 <- cells_AUC[,100:140]
cbind(sample1, sample2)