Description Usage Arguments Value Objects from the Class Slots Extends Methods Display Parameters Author(s) See Also Examples
DEPRECATED! This class and methods are deprecated, please use DataTrack
,
AnnotationTrack
or AlignmentsTrack
instead.
A class to represent short sequences that have been aligned to a reference genome as they are typically generated in a next generation sequencing experiment.
1 2 |
range |
An object of class
Alternatively, the |
start, end, width |
Integer vectors, giving the start and the end end coordinates for the individual track items, or their width. Two of the three need to be specified, and have to be of equal length or of length one, in which case this value will be recycled. Otherwise, the usual R recycling rules for vectors do not apply. |
strand |
Character vector, the strand information for the
individual track items. Needs to be of equal length as the
|
chromosome |
The chromosome on which the track's genomic ranges
are defined. A valid UCSC chromosome identifier. Please note that at
this stage only syntactic checking takes place, i.e., the argument
value needs to be a single integer, numeric character or a character
of the form |
genome |
The genome on which the track's ranges are defined. Usually this is a valid UCSC genome identifier, however this is not being formally checked at this point. |
stacking |
The stacking type for overlapping items of the
track. One in |
name |
Character scalar of the track's name used in the title panel when plotting. |
coverageOnly |
Instead of storing individual reads, just compute the coverage and store the resulting coverage vector. |
... |
Additional items which will all be interpreted as further display parameters. |
The return value of the constructor function is a new object of class
AlignedReadTrack
.
Objects can be created using the constructor function AlignedReadTrack
.
coverage
:Object of class "list"
, a list of
coverage vectors for the plus strand, the minus strand and for
both strands combined.
coverageOnly
:Object of class "logical"
, flag
to determine whether the object stores read locations or the coverage
vectors only.
stacking
:Object of class "character"
,
inherited from class StackedTrack
stacks
:Object of class "environment"
,
inherited from class StackedTrack
range
:Object of class GRanges
,
inherited from class RangeTrack
chromosome
:Object of class "character"
,
inherited from class RangeTrack
genome
:Object of class "character"
, inherited
from class RangeTrack
dp
:Object of class
DisplayPars
, inherited from class
GdObject
name
:Object of class "character"
, inherited
from class GdObject
imageMap
:Object of class
ImageMap
, inherited from class
GdObject
Class "StackedTrack"
, directly.
Class "RangeTrack"
, by class "StackedTrack",
distance2.
Class "GdObject"
, by class "StackedTrack",
distance3.
In the following code chunks, obj
is considered to be an object
of class AlignedReadTrack
.
Exported in the name space:
signature(x="AlignedReadTrack")
: subset the items in
the AlignedReadTrack
. This is essentially similar to
subsetting of the GRanges
object in the
range
slot. For most applications, the subset
method
may be more appropriate. The operation is only supported for
objects that still contain all the read locations, i.e.,
coverageOnly=FALSE
.
Additional Arguments:
i
: subsetting indices
Examples:
obj[1:5]
signature(x="AlignedReadTrack")
: subset a
AlignedReadTrack
by coordinates and sort if necessary.
Usage:
subset(x, from, to, sort=FALSE, stacks=FALSE)
Additional Arguments:
from
, to
: the coordinates range to subset
to.
sort
: sort the object after subsetting. Usually
not necessary.
stacks
: recompute the stacking after subsetting
which can be expensive and is not always necessary.
Examples:
subset(obj, from=10, to=20)
subset(obj, from=10, to=20, sort=TRUE, stacks=FALSE)
signature(x="AlignedReadTrack")
: split an
AlignedReadTrack
object by an appropriate factor vector (or
another vector that can be coerced into one). The output of this
operation is a list of AlignedReadTrack
objects.
Additional Arguments:
f
: the splitting factor.
...
: all further arguments are ignored.
Usage:
split(x, f, ...)
Examples:
split(obj, c("a", "a", "b", "c", "a"))
signature(x="AlignedReadTrack")
: return the
coverage vector for one of the strands, or the combined vector.
Usage:
coverage(x, strand="*")
Additional Arguments:
strand
: the selector for the strand, +
for
the Watson strand, -
for the Crick strand or *
for both strands.
Examples:
coveraget(obj)
coverage(obj, strand="-")
Internal methods:
signature(GdObject="AlignedReadTrack")
:
recompute the coverage on the plus and minus strand as well as for
the combined strands and update the respective slot.
Usage:
setCoverage(GdObject)
Examples:
setCoverage(obj)
signature(GdObject="AlignedReadTrack")
: add a y-axis
to the title panel of a track.
Usage:
drawAxis(GdObject, from, to, subset=FALSE, ...)
Additional Arguments:
from
, to
: compute axis range from the data
within a certain coordinates range only.
subset
: subset the object prior to calculating
the axis ranges. Can be expensive and is not always needed.
...
: all further arguments are ignored.
Examples:
Gviz:::drawAxis(obj)
signature(gdObject="AlignedReadTrack")
: plot the
object to a graphics device. The return value of this method is
the input object, potentially updated during the plotting
operation. Internally, there are two modes in which the method can
be called. Either in 'prepare' mode, in which case no plotting is
done but the object is preprocessed based on the
available space, or in 'plotting' mode, in which case the actual
graphical output is created. Since subsetting of the object can be
potentially costly, this can be switched off in case subsetting
has already been performed before or is not necessary.
Usage:
drawGD(GdObject, minBase, maxBase, prepare=FALSE,
subset=TRUE, ...)
Additional Arguments:
minBase
, maxBase
: the coordinate range to
plot.
prepare
: run method in preparation or in
production mode.
subset
: subset the object to the visible region
or skip the potentially expensive subsetting operation.
...
: all further arguments are ignored.
Examples:
Gviz:::drawGD(obj)
Gviz:::drawGD(obj, minBase=1, maxBase=100)
Gviz:::drawGD(obj, prepare=TRUE,
subset=FALSE)
signature(GdObject="AlignedReadTrack")
: superpose a grid on top of a track.
Usage:
drawGrid(GdObject, from, to)
Additional Arguments:
from
, to
: draw grid within a certain
coordinates range. This needs to be supplied for the plotting
function to know the current genomic coordinates.
Examples:
Gviz:::drawGrid(obj, from=10, to=100)
signature(.Object="AligendReadTrack")
:
initialize the object.
signature(object="AlignedReadTrack")
: show a
human-readable summary of the object.
Inherited methods:
signature(GdObject="AlignedReadTrack")
: return
the current stacking type.
Usage:
stacking(GdObject)
Examples:
stacking(obj)
signature(GdObject="AlignedReadTrack",
value="character")
: set the object's stacking type to one in
c(hide, dense, squish, pack,full)
.
Usage:
stacking<-(GdObject, value)
Additional Arguments:
value
: replacement value.
Examples:
stacking(obj) <- "squish"
signature(GdObject="AlignedReadTrack")
:
recompute the stacks based on the available space and on the
object's track items and stacking settings.
Usage:
setStacks(GdObject, from, to)
Additional Arguments:
from
, to
: compute stacking within a
certain coordinates range. This needs to be supplied for the
plotting function to know the current genomic coordinates.
Examples:
Gviz:::setStacks(obj)
signature(GdObject="AlignedReadTrack")
: return
the stack indices for each track item.
Usage:
stacks(GdObject)
Examples:
Gviz:::stacks(obj)
signature(GdObject="AlignedReadTrack")
:
return the chromosome for which the track is defined.
Usage:
chromosome(GdObject)
Examples:
chromosome(obj)
signature(GdObject="AlignedReadTrack")
:
replace the value of the track's chromosome. This has to be a
valid UCSC chromosome identifier or an integer or character
scalar that can be reasonably coerced into one.
Usage:
chromosome<-(GdObject, value)
Additional Arguments:
value
: replacement value.
Examples:
chromosome(obj) <- "chr12"
signature(x="AlignedReadTrack")
: the
start or end coordinates of the track items, or their width in
genomic coordinates.
Usage:
start(x)
end(x)
width(x)
Examples:
start(obj)
end(obj)
width(obj)
signature(x="AlignedReadTrack")
:
replace the start or end coordinates of the track items, or their
width.
Usage:
start<-(x, value)
end<-(x, value)
width<-(x, value)
Additional Arguments:
value
: replacement value.
Examples:
start(obj) <- 1:10
end(obj) <- 20:30
width(obj) <- 1
signature(GdObject="AlignedReadTrack")
: the
arithmetic mean of the track item's coordionates, i.e.,
(end(obj)-start(obj))/2
.
Usage:
position(GdObject)
Examples:
position(obj)
signature(GdObject="AlignedReadTrack")
: return the
grouping information for track items. For certain sub-classes,
groups may be indicated by different color schemes when
plotting. See grouping
or
AnnotationTrack
and
GeneRegionTrack
for details.
Usage:
feature(GdObject)
Examples:
feature(obj)
signature(gdObject="AlignedReadTrack",
value="character")
: set the grouping information for track
items. This has to be a factor vector (or another type of vector
that can be coerced into one) of the same length as the number of
items in the AlignedReadTrack
. See grouping
or
AnnotationTrack
and
GeneRegionTrack
for details.
Usage:
feature<-(GdObject, value)
Additional Arguments:
value
: replacement value.
Examples:
feature(obj) <- c("a", "a", "b", "c", "a")
signature(x="AlignedReadTrack")
: return the track's genome.
Usage:
genome(x)
Examples:
genome(obj)
signature(x="AlignedReadTrack")
: set the track's
genome. Usually this has to be a valid UCSC identifier, however
this is not formally enforced here.
Usage:
genome<-(x, value)
Additional Arguments:
value
: replacement value.
Examples:
genome(obj) <- "mm9"
signature(x="AlignedReadTrack")
: return the number
of items in the track.
Usage:
length(x)
Examples:
length(obj)
signature(x="AlignedReadTrack")
: return the genomic
coordinates for the track as an object of class
IRanges
.
Usage:
range(x)
Examples:
range(obj)
signature(x="AlignedReadTrack")
: return the genomic
coordinates for the track along with all additional annotation
information as an object of class GRanges
.
Usage:
ranges(x)
Examples:
ranges(obj)
signature(x="AlignedReadTrack")
: return a vector of
strand specifiers for all track items, in the form '+' for the
Watson strand, '-' for the Crick strand or '*' for either of the
two.
Usage:
strand(x)
Examples:
strand(obj)
signature(x="AlignedReadTrack")
: replace the
strand information for the track items. The replacement value
needs to be an appropriate scalar or vector of strand values.
Usage:
strand<-(x, value)
Additional Arguments:
value
: replacement value.
Examples:
strand(obj) <- "+"
signature(x="AlignedReadTrack")
: return all
additional annotation information except for the genomic coordinates
for the track items as a data.frame.
Usage:
values(x)
Examples:
values(obj)
signature(from="AlignedReadTrack",
to="data.frame")
: coerce the GRanges
object in the range
slot into a regular data.frame.
Examples:
as(obj, "data.frame")
signature(x="AlignedReadTrack",
name="character")
: list the value of the display parameter
name
. See settings
for details on display
parameters and customization.
Usage:
displayPars(x, name)
Examples:
displayPars(obj, "col")
signature(x="AlignedReadTrack",
name="missing")
: list the value of all available display
parameters. See settings
for details on display
parameters and customization.
Examples:
displayPars(obj)
signature(x="AlignedReadTrack", name="character")
:
alias for the displayPars
method. See
settings
for details on display parameters and
customization.
Usage:
getPar(x, name)
Examples:
getPar(obj, "col")
signature(x="AlignedReadTrack", name="missing")
:
alias for the displayPars
method. See
settings
for details on display parameters and
customization.
Examples:
getPar(obj)
signature(x="AlignedReadTrack",
value="list")
: set display parameters using the values of the
named list in value
. See settings
for details
on display parameters and customization.
Usage:
displayPars<-(x, value)
Examples:
displayPars(obj) <- list(col="red", lwd=2)
signature(x="AlignedReadTrack", value="character")
:
set the single display parameter name
to value
. Note
that display parameters in the AlignedReadTrack
class are
pass-by-reference, so no re-assignmnet to the symbol obj
is
necessary. See settings
for details on display
parameters and customization.
Usage:
setPar(x, name, value)
Additional Arguments:
name
: the name of the display parameter to set.
Examples:
setPar(obj, "col", "red")
signature(x="AlignedReadTrack", value="list")
: set
display parameters by the values of the named list in
value
. Note that display parameters in the
AlignedReadTrack
class are pass-by-reference, so no
re-assignmnet to the symbol obj
is necessary. See
settings
for details on display parameters and
customization.
Examples:
setPar(obj, list(col="red", lwd=2))
signature(GdObject="AlignedReadTrack")
: return
grouping information for the individual items in the track. Unless
overwritten in one of the sub-classes, this usualy returns
NULL
.
Usage:
group(GdObject)
Examples:
group(obj)
signature(x="AlignedReadTrack")
: return the value of
the name
slot.
Usage:
names(x)
Examples:
names(obj)
signature(x="AlignedReadTrack", value="character")
:
set the value of the name
slot.
Usage:
names<-(x, value)
Examples:
names(obj) <- "foo"
signature(ImageMap="AlignedReadTrack")
: return the
coordinates from the internal image map.
Usage:
coords(ImageMap)
Examples:
coords(obj)
signature(x="AlignedReadTrack")
: return the tags from the
internal image map.
Usage:
tags(x)
Examples:
tags(obj)
The following display parameters are set for objects of class
AlignedReadTrack
upon instantiation, unless one or more of
them have already been set by one of the optional sub-class initializers,
which always get precedence over these global defaults. See
settings
for details on setting graphical parameters
for tracks.
collapse=FALSE
: collapse overlapping ranges and
aggregate the underlying data.
detail="coverage"
: the amount of detail to plot the
data. Either coverage
to show the coverage only, or
reads
to show individual reads. For large data sets the
latter can be very inefficient. Please note that reads
is
only available when the object has been created with option
coverageOnly=FALSE
.
fill="#0080ff"
: the fill color for the coverage
indicator.
size=NULL
: the relative size of the track. Defaults
to size selection based on the underlying data. Can be overridden
in the plotTracks
function.
type="histogram"
: the plot type, one or several in
c("p","l", "b", "a", "s", "g", "r", "S", "smooth", "histogram",
"mountain", "polygon", "h", "boxplot", "gradient", "heatmap",
"horizon")
. See the 'Details' section in DataTrack
for more information on the individual plotting types.
Additional display parameters are being inherited from the respective
parent classes. Note that not all of them may have an effect on the
plotting of AlignedReadTrack
objects.
StackedTrack
:
reverseStacking=FALSE
: Logical flag. Reverse
the y-ordering of stacked items. I.e., features that are
plotted on the bottom-most stacks will be moved to the top-most
stack and vice versa.
stackHeight=0.75
: Numeric between 0 and 1.
Controls the vertical size and spacing between stacked
elements. The number defines the proportion of the total
available space for the stack that is used to draw the glyphs.
E.g., a value of 0.5 means that half of the available vertical
drawing space (for each stacking line) is used for the glyphs,
and thus one quarter of the available space each is used for
spacing above and below the glyph. Defaults to 0.75.
GdObject
:
alpha=1
: Numeric scalar. The transparency for
all track items.
alpha.title=NULL
: Numeric scalar. The transparency
for the title panel.
background.legend="transparent"
: Integer or
character scalar. The background color for the legend.
background.panel="transparent"
: Integer or
character scalar. The background color of the content panel.
background.title="lightgray"
: Integer or character
scalar. The background color for the title panel.
cex=1
: Numeric scalar. The overall font expansion
factor for all text and glyphs, unless a more specific
definition exists.
cex.axis=NULL
: Numeric scalar. The expansion
factor for the axis annotation. Defaults to NULL
, in
which case it is automatically determined based on the
available space.
cex.title=NULL
: Numeric scalar. The expansion
factor for the title panel. This effects the fontsize of both
the title and the axis, if any. Defaults to NULL
,
which means that the text size is automatically adjusted to
the available space.
col="#0080FF"
: Integer or character scalar.
Default line color setting for all plotting elements, unless
there is a more specific control defined elsewhere.
col.axis="white"
: Integer or character scalar.
The font and line color for the y axis, if any.
col.border.title="white"
: Integer or character
scalar. The border color for the title panels.
col.frame="lightgray"
: Integer or character
scalar. The line color used for the panel frame, if
frame==TRUE
col.grid="#808080"
: Integer or character scalar.
Default line color for grid lines, both when type=="g"
in DataTrack
s and when display parameter
grid==TRUE
.
col.line=NULL
: Integer or character scalar.
Default colors for plot lines. Usually the same as the global
col
parameter.
col.symbol=NULL
: Integer or character scalar.
Default colors for plot symbols. Usually the same as the
global col
parameter.
col.title="white"
(Aliases: fontcolor.title)
:
Integer or character scalar. The border color for the title panels
fontcolor="black"
: Integer or character scalar.
The font color for all text, unless a more specific definition
exists.
fontface=1
: Integer or character scalar. The
font face for all text, unless a more specific definition exists.
fontface.title=2
: Integer or character scalar.
The font face for the title panels.
fontfamily="sans"
: Integer or character scalar.
The font family for all text, unless a more specific definition
exists.
fontfamily.title="sans"
: Integer or character
scalar. The font family for the title panels.
fontsize=12
: Numeric scalar. The font size for
all text, unless a more specific definition exists.
frame=FALSE
: Boolean. Draw a frame around the
track when plotting.
grid=FALSE
: Boolean, switching on/off the plotting
of a grid.
h=-1
: Integer scalar. Parameter controlling the
number of horizontal grid lines, see panel.grid
for details.
lineheight=1
: Numeric scalar. The font line
height for all text, unless a more specific definition exists.
lty="solid"
: Numeric scalar. Default line type
setting for all plotting elements, unless there is a more
specific control defined elsewhere.
lty.grid="solid"
: Integer or character scalar.
Default line type for grid lines, both when type=="g"
in DataTrack
s and when display parameter
grid==TRUE
.
lwd=1
: Numeric scalar. Default line width setting
for all plotting elements, unless there is a more specific
control defined elsewhere.
lwd.border.title=1
: Integer scalar. The border
width for the title panels.
lwd.grid=1
: Numeric scalar. Default line width
for grid lines, both when type=="g"
in DataTrack
s
and when display parameter grid==TRUE
.
lwd.title=1
: Integer scalar. The border width
for the title panels
min.distance=1
: Numeric scalar. The minimum
pixel distance before collapsing range items, only if
collapse==TRUE
. See collapsing
for details.
min.height=3
: Numeric scalar. The minimum range
height in pixels to display. All ranges are expanded to this
size in order to avoid rendering issues. See collapsing
for details.
min.width=1
: Numeric scalar. The minimum range
width in pixels to display. All ranges are expanded to this
size in order to avoid rendering issues. See collapsing
for details.
reverseStrand=FALSE
: Logical scalar. Set up the
plotting coordinates in 3' -> 5' direction if TRUE
.
This will effectively mirror the plot on the vertical axis.
rotation=0
: The rotation angle for all text
unless a more specific definiton exists.
rotation.title=90
(Aliases: rotation.title)
:
The rotation angle for the text in the title panel. Even
though this can be adjusted, the automatic resizing of the
title panel will currently not work, so use at own risk.
showAxis=TRUE
: Boolean controlling whether to
plot a y axis (only applies to track types where axes are
implemented).
showTitle=TRUE
: Boolean controlling whether to
plot a title panel. Although this can be set individually
for each track, in multi-track plots as created by
plotTracks
there will still be an empty
placeholder in case any of the other tracks include a title.
The same holds true for axes. Note that the the title panel
background color could be set to transparent in order to
completely hide it.
v=-1
: Integer scalar. Parameter controlling the
number of vertical grid lines, see panel.grid
for details.
Florian Hahne
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 | ## This class is deprecated!
## Please use instead:
## - DataTrack
## - AnnotationTrack
## - AlignmentsTrack
## Below example from AnnotationTrack
## An empty object
AnnotationTrack()
## Construct from individual arguments
st <- c(2000000, 2070000, 2100000, 2160000)
ed <- c(2050000, 2130000, 2150000, 2170000)
str <- c("-", "+", "-", "-")
gr <- c("Group1","Group2","Group1", "Group3")
annTrack <- AnnotationTrack(start=st, end=ed, strand=str, chromosome=7,
genome="hg19", feature="test", group=gr,
id=paste("annTrack item", 1:4),
name="generic annotation", stacking="squish")
## Plotting
plotTracks(annTrack)
|
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