normalizePlateEffect: Normalization of plate effects

Description Usage Arguments Details Value Author(s) See Also Examples

View source: R/normalization.R

Description

Normalization of plate effects in the screen.

Usage

1
normalizePlateEffect(sgi, type = "Bscore", maxit = 20, verbose = 0)

Arguments

sgi

An object of class RNAinteract

type

If type is "Bscore" (default) a Bscore-normalization is performed. If type is "spatial", a locfit regression is estimated that accounts for spatial effects.

maxit

Maximum number of iterations for locfit.

verbose

Either 0 (default, no output), 1 (minimum output), or 2 (outout).

Details

The Bscore normalization estimates row and column effects for each plate. It returns the residuals to the sum of row and column effects. The spatial normalization estimates a non-linear 2D regression for each plate and returns the residuals.

Value

An object of class RNAinteract. The returned object contains the normalization information.

Author(s)

Bernd Fischer

See Also

RNAinteract-package

Examples

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2

RNAinteract documentation built on Nov. 8, 2020, 5:28 p.m.