Description Usage Arguments Details Value References Examples
Corrects the PM intensities in a ProbeSet
for non-specific binding.
1 2 3 4 5 | pmcorrect.pmonly(object)
pmcorrect.subtractmm(object)
pmcorrect.mas(object, contrast.tau=0.03, scale.tau=10, delta=2^(-20))
|
object |
An object of class |
contrast.tau |
a number denoting the contrast tau parameter in the MAS 5.0 pm correction algorithm. |
scale.tau |
a number denoting the scale tau parameter in the MAS 5.0 pm correction algorithm. |
delta |
a number denoting the delta parameter in the MAS 5.0 pm correction algorithm. |
These are the pm correction methods perfromed by Affymetrix MAS 4.0 (subtractmm) and MAS 5.0 (mas). See the Affymetrix Manual for details. pmonly does what you think: does not change the PM values.
A ProbeSet
for which the
pm
slot contains the corrected PM values.
Affymetrix MAS 4.0 and 5.0 manual
1 2 3 4 5 6 7 8 9 | if (require(affydata)) {
data(Dilution)
gn <- geneNames(Dilution)
pps <- probeset(Dilution, gn[1])[[1]]
pps.pmonly <- pmcorrect.pmonly(pps)
pps.subtractmm <- pmcorrect.subtractmm(pps)
pps.mas5 <- pmcorrect.mas(pps)
}
|
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