generateExprSet-methods: generate a set of expression values

Description Usage Arguments Details See Also Examples

Description

Generate a set of expression values from the probe pair information. The set of expression is returned as an ExpressionSet object.

Usage

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  computeExprSet(x, pmcorrect.method, summary.method, ...)

  generateExprSet.methods()

  upDate.generateExprSet.methods(x)

Arguments

x

a AffyBatch holding the probe level informations to generate the expression values, for computeExprSet, and for upDate.generateExprSet.methods it is a character vector..

pmcorrect.method

the method used to correct PM values (see section 'details').

summary.method

the method used to generate the expression value (see section 'details').

...

any of the options of the normalization you would like to modify.

Details

An extra argument ids= can be passed. It must be a vector of affids. The expression values will only be computed and returned for these affyids.

The different methods available through this mechanism can be accessed by calling the method generateExprSet.methods with an object of call Cel.container as an argument.

In the Affymetrix design, MM probes were included to measure the noise (or background signal). The original algorithm for background correction was to subtract the MM signal to the PM signal. The methods currently included in the package are "bg.correct.subtractmm", "bg.correct.pmonly" and "bg.correct.adjust".

To alter the available methods for generating ExprSets use upDate.generateExprSet.methods.

See Also

method generateExprSet of the class AffyBatch
expresso

Examples

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if (require(affydata)) {
  data(Dilution)

  ids <- c( "1000_at","1001_at")

  eset <- computeExprSet(Dilution, pmcorrect.method="pmonly",
                         summary.method="avgdiff", ids=ids)
}

Example output

Loading required package: BiocGenerics
Loading required package: parallel

Attaching package:BiocGenericsThe following objects are masked frompackage:parallel:

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked frompackage:stats:

    IQR, mad, sd, var, xtabs

The following objects are masked frompackage:base:

    anyDuplicated, append, as.data.frame, basename, cbind, colnames,
    dirname, do.call, duplicated, eval, evalq, Filter, Find, get, grep,
    grepl, intersect, is.unsorted, lapply, Map, mapply, match, mget,
    order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank,
    rbind, Reduce, rownames, sapply, setdiff, sort, table, tapply,
    union, unique, unsplit, which.max, which.min

Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: affydata
     Package    LibPath                   Item      
[1,] "affydata" "/usr/lib/R/site-library" "Dilution"
     Title                        
[1,] "AffyBatch instance Dilution"
2 ids to be processed

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|####################|
Warning messages:
1: replacing previous importAnnotationDbi::tailbyutils::tailwhen loadinghgu95av2cdf2: replacing previous importAnnotationDbi::headbyutils::headwhen loadinghgu95av2cdf

affy documentation built on Nov. 8, 2020, 8:18 p.m.