add_error: add sequencing error to simulated reads

In polyester: Simulate RNA-seq reads

Description

simulate sequencing error by randomly changing the sequenced nucleotide on some of the reads

Usage

add_error(tFrags, error_rate = 0.005)

Arguments

tFrags	DNAStringSet representing sequencing reads
error_rate	error probability

Value

DNAStringSet equivalent to tFrags but with random sequencing errors inserted

Examples

  library(Biostrings)
  data(srPhiX174)
  set.seed(174)
  srPhiX174_withError = add_error(srPhiX174)
  #error was introduced in, e.g., position 10 of 2nd string in set.

polyester documentation built on Nov. 8, 2020, 8:09 p.m.