model5: Empirical error model for Illumina Genome Analyzer IIx with...

In polyester: Simulate RNA-seq reads

Description

for each position in mate 2 of a paired-end read generated with the specified Illumina chemistry, this data frame contains the probability of not making a sequencing error, and of making each of the 4 possible types of sequencing errors. The reference base (truth) is in column 1, and the probabilities of sequencing that base given its read position (column 7) as each of the 5 possible bases (A, T, G, C, and N) is given in columns 2 through 6, respectively. So for example, at position 8 in mate 1 of a read where the true base is A, the probability of correctly calling that base an A is 0.9992, the probability of making an error by sequencing a T is 0.0002, the probability of making an error by sequencing a G is 0.0002, the probability of making an error by sequencing a C is 0.0001, and the probability of reading an 'N' at position 8 is 0.0002. This can be seen by looking at model5[model5$pos == 8,]. Note that position indexing is 1-based, though a 0 position is included as described in the GemSIM documentation.

Format

data frame named model5, 7 columns, 505 rows

Source

processed from the Illumina v5 error model that ships with GemSIM (see references)

References

McElroy KE, Luciani F, Thomas T (2012). GemSIM: general, error-model based simulator of next-generation sequencing data. BMC Genomics 13(1), 74.

polyester documentation built on Nov. 8, 2020, 8:09 p.m.