Propagate probe-level uncertainty in model-based clustering on gene expression data

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Description

This function clusters gene expression using a Gaussian mixture model including probe-level measurement error. The inputs are gene expression levels and the probe-level standard deviation associated with expression measurement for each gene on each chip. The outputs is the clustering results.

Usage

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pumaClust(e=NULL, se=NULL, efile=NULL, sefile=NULL, 
      subset=NULL, gsnorm=FALSE, clusters, 
      iter.max=100, nstart=10, eps=1.0e-6, del0=0.01)

Arguments

e

either a valid ExpressionSet object, or a data frame containing the expression level for each gene on each chip.

se

data frame containing the standard deviation of gene expression levels.

efile

character, the name of the file which contains gene expression measurements.

sefile

character, the name of the file which contains the standard deviation of gene expression measurements.

subset

vector specifying the row number of genes which are clustered on.

gsnorm

logical specifying whether do global scaling normalisation or not.

clusters

integer, the number of clusters.

iter.max

integer, the maximum number of iterations allowed in the parameter initialisation.

nstart

integer, the number of random sets chosen in the parameter initialisation.

eps

numeric, optimisation parameter.

del0

numeric, optimisation parameter.

Details

The input data is specified either as an ExpressionSet object (in which case se, efile and sefile will be ignored), or by e and se, or by efile and sefile.

Value

The result is a list with components

cluster: vector, containing the membership of clusters for each gene; centers: matrix, the center of each cluster; centersigs: matrix, the center variance of each cluster; likelipergene: matrix, the likelihood of belonging to each cluster for each gene; bic: numeric, the Bayesian Information Criterion score.

Author(s)

Xuejun Liu, Magnus Rattray

References

Liu,X., Lin,K.K., Andersen,B., and Rattray,M. (2006) Propagating probe-level uncertainty in model-based gene expression clustering, BMC Bioinformatics, 8(98).

Liu,X., Milo,M., Lawrence,N.D. and Rattray,M. (2005) A tractable probabilistic model for Affymetrix probe-level analysis across multiple chips, Bioinformatics, 21(18):3637-3644.

See Also

Related method mmgmos and pumaClustii

Examples

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