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# ______________________________________________________________________
# <RCircos DEMO><RCircos DEMO><RCircos DEMO><RCircos DEMO><RCircos DEMO>
#
# This demo draw chromosome ideogram with padding between chromosomes,
# highlights, chromosome names, and histogram.
#
# Usage:
#
# library(RCircos);
# demo("RCircos.Histogram.Demo");
# ______________________________________________________________________
# <RCircos DEMO><RCircos DEMO><RCircos DEMO><RCircos DEMO><RCircos DEMO>
# Load RCircos package and defined parameters
# ________________________________________________________
# xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
library(RCircos);
# Load human cytoband data and gene expression data
# ________________________________________________________
# xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
data(RCircos.Histogram.Data);
data(UCSC.HG19.Human.CytoBandIdeogram);
cyto.info <- UCSC.HG19.Human.CytoBandIdeogram;
# Setup RCircos core components:
# _________________________________________________________
# xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
RCircos.Set.Core.Components(cyto.info, NULL, 10, 0);
# Open the graphic device (here a pdf file)
# _________________________________________________________
# xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
out.file <- "RCircos.Histogram.Demo.pdf";
pdf(file=out.file, height=8, width=8);
RCircos.Set.Plot.Area();
# Draw chromosome ideogram
# _________________________________________________________
# xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
message("Draw chromosome ideogram ...\n");
RCircos.Chromosome.Ideogram.Plot();
title("RCircos Histogram Plot Demo");
# Plot histogram Inside of chromosome ideogram
# _________________________________________________________
# xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
hist.data <- RCircos.Histogram.Data;
hist.colors <- rep("blue", nrow(hist.data))
rows <- which(hist.data$Data>0.4)
hist.colors[rows] <- "red";
hist.data["PlotColor"] <- hist.colors;
data.col <- 4;
track.num <- 1;
RCircos.Histogram.Plot(hist.data, data.col, track.num, "in");
# Close the graphic device and clear memory
# _________________________________________________________
# xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
dev.off();
message("RCircos Histogram Demo Done!");
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