Filters the dataset based upon signal from the specified columns. Can be normalized to the average signal of any given peptide at the given concentration. Works for multiple RFU signal inputs or a single Kd input.
An R object, usually a
An integer sequence, defines the columns that contain the concentration data to be used
for hits selection. A given peptide will have to qualify as a hit at all given
concentration columns to be considered a true peptide hit. Ex: Hits based
upon 3 concentrations in columns 5 through 8 = 5:8. If
A character string that defines the peptide to to normalize to. Hits must be 5 times
higher in signal than the given peptide to be returned as hits. Normally "GSG".If
Toggle that determines if hits will be selected by RFU signal or Kd values.
data.frame will be returned only with the peptides that are hits in the given
context. (Hits must have Avg signal 5 times greater than the average signal of the peptide
specified in the argument
Cutoff. Or one fifth (0.2) the
Cutoff Kd value if
Kd = TRUE)
1 2 3 4 5 6 7 8 9 10 11
protEx.hitSel <- data.frame(Peptides = c("PWRGPWARVGSG","GYNRVGQGSG","PNGYRSGVKGSG","GSG"), Kd = c(0.2572361,2.8239730,3.3911868,281.3058),C_6uM = c(65011.48,47462.24,24778,2613.03), C_3uM = c(62637.81,31899.85,21313.67,1161.216),C_1.5uM = c(57893.22,25911.35,10397.99,630.4025)) ## Hits selection by RFU signal ## hitSelRFU <- hitSel(protEx.hitSel,3:5,"GSG",Kd = FALSE) ## Hits selection by calculated Kd ## hitSelKd <- hitSel(protEx.hitSel,2,"GSG",Kd = TRUE)
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