Calculates the mean with standard error, and population peptides at each length/charge combination
within a VDAP dataset. If the argument
Glob = TRUE, average signals will be compared against
a global set of peptides and p - values will be calculated for hypoethesis testing.
will also return a plot for visualization of signal, population, and hypothesis testing.
An R object, usually a
The column contianing the concentration or Kd data to be analyzed, an integer. Default is column 5
which is generally the highest concentration average according to the default formatting function
Ex: Column 1 = 1
Toggle to calculate by a defined signal column or by calculated Kd values, effects final plot behavior
and labels. If
data.frame will be returned with columns for the mean, standard error,
and population of peptides at each length/charge combination that can be exported
for further analysis. Also uitilizes ggplot2 and reshape2 to create
a heat map plot that shows the signal distribution with corresponding populations that
can be exported.
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protEx.lcScan <- data.frame(Peptides = c("PWRGPWARVGSG","GYNRVGQGSG","PNGYRSGVKGSG","GSG"), Length = c(12,10,12,3),Charge = c(2,1,2,0),Kd = c(0.2572361,2.8239730,3.3911868,281.3058), C_6uM = c(65011.48,47462.24,24778,2613.03),C_6uM2 = c(62637.81,20723.85,21313.67,2300.216)) ## Signal length/charge Analysis ## lcScanEx <- lcScan(protEx.lcScan) ## Kd length/charge Analysis ## lcScanEx <- lcScan(protEx.lcScan, Conc = 4, Kd = TRUE)
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