Description Usage Arguments Details Value Author(s) See Also Examples
Genotype calls represented as numeric values (allele ratios) within [0, 1] are converted to character strings containing allele information “A”, “T”, “C”, and “G”
1 2 3 4 5 6 | translateTheta(calls, resInfo, type = "regular")
translateThetaCombined(BSRed, mergedCalls = NULL)
translateThetaFromFiles(dataFiles, mergedCalls = NULL,
markerStep = 1000, sep = "\t", quote = "")
|
calls |
Numeric matrix with calls {0, 1/2, 1} representing allele ratios for each
sample. Each row is a unique marker or paralogue (specified with |
resInfo |
Data table containing |
type |
One of “regular”, “single”, or “merged” (see details below) |
BSRed |
|
mergedCalls |
Matrix with calls from resolved MSV-5 paralogs (see
|
dataFiles |
Character vector containing file names (see |
markerStep |
The maximum number of markers loaded into the workspace at the time |
sep |
Field delimiter in text-files (see |
quote |
Quote-marks used for character strings (see |
The main difference between translateTheta
and
translateThetaCombined
is that the former can only handle
call-values {0, 1/2, 2} whereas the latter handles values
{0, 1/4, 1/2, 3/4, 1}. In effect this means that markers from
duplicated genome regions have to be handled in a special way if
analysed with translateTheta
. If type == "regular"
, the
markers are treated as if they were all from
a diploid region. This implies that all non-segregating paralogs of
“MSV-a” and “MSV-b” markers are ignored, effectively
turning these markers into SNPs. Markers classified as
“MSV-5” or “PSV” are set to missing (see
makeDiploidCalls
). If type == "single"
,
calls
is expected to contain resolved “MSV-5” paralogs named
with “-Para1” or “-Para2” (see
unmixParalogues
). If type == "merged"
,
resolved “MSV-5” paralogs named according to their respective
chromosomes, “-ChromX”, are expected (see
assignParalogues
). The main use of this function is to
prepare genotype calls for mapping software which requires diploid
markers.
With translateThetaCombined
, there is always one element per
marker, as required by the "AlleleSetIllumina"
. If
mergedCalls
is given, the “MSV-5” paralogs will be
resolved, otherwise only the ratio of the alleles across paralogs will
be returned. The function translateThetaFromFiles
performs the
same operations on data sequentially loaded into the workspace, and
the genotypes are written to file dataFiles$genoFile
as they
are found.
Output from translateTheta
is a matrix whose dimensions depend
on the input data. If calls
has one row per marker (i.e. type
== "regular"
), the number of rows in the output matrix also
equals the number of markers. If calls
has one row per
paralogue (i.e. type != "regular"
), the number of rows
in the output matrix also equals the number of paralogs. Each element
is a character string “x y” denoting the two alleles
(“A”, “T”, “C”, “G”, or “-” for
missing).
In contrast, the output from translateThetaCombined
is an
AlleleSetIllumina
object with an added assayData
entry “genotype”. The elements of this matrix representing
diploid markers are given as “xy”, un-resolved tetraploid markers
are given as “xyzw”, and resolved tetraploid markers are given
as “xy,zw” (paralogs separated by comma). The letters
correspond to any of the 4 bases or “-” for missing.
The function translateThetaFromFiles
are used for its side
effects.
Lars Gidskehaug
makeDiploidCalls
, unmixParalogues
,
assignParalogues
, makeFilenames
,
callGenotypes
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 | ## Not run:
#Read 25 markers into an AlleleSetIllumina object
rPath <- system.file("extdata", package="beadarrayMSV")
normOpts <- setNormOptions()
dataFiles <- makeFilenames('testdata',normOpts,rPath)
beadFile <- paste(rPath,'beadData_testdata.txt',sep='/')
beadInfo <- read.table(beadFile,sep='\t',header=TRUE,as.is=TRUE)
BSRed <- createAlleleSetFromFiles(dataFiles[1:4],markers=1:25,beadInfo=beadInfo)
#Genotype calling
BSRed <- callGenotypes(BSRed)
genotypes <- translateTheta(assayData(BSRed)$call,fData(BSRed),type='regular')
print(cbind(fData(BSRed)$Classification,genotypes[,1:3])[1:10,])
#Alternative output
BSRed <- translateThetaCombined(BSRed)
print(cbind(fData(BSRed)$Classification,assayData(BSRed)$genotype[,1:3])[1:10,])
## End(Not run)
|
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