reclassify_droplets: Analysis step: Reclassify droplets

In ddpcr: Analysis and Visualization of Droplet Digital PCR in R and on the Web

Analysis step: Reclassify droplets

Description

After classifying droplets into clusters in each well individually, it may be useful to attempt to reclassify droplets in wells where the gates are not very clearly defined. This function uses information taken from wells with a high negative frquency (where the gate is easily defined) to adjust the gates in the other wells.

See the README for more information about the algorithm used.

Usage

reclassify_droplets(plate)

Details

This function is recommended to be run as part of an analysis pipeline (ie. within the analyze function) rather than being called directly.

Note

This is an S3 generic, which means that different ddPCR plate types can implement this function differently. See the README for more information on how to implement custom ddPCR plate types.

See Also

analyze
reclassify_droplets_single
mark_clusters

ddpcr documentation built on Aug. 21, 2023, 1:07 a.m.