remove_empty: Analysis step: Remove empty droplets

In ddpcr: Analysis and Visualization of Droplet Digital PCR in R and on the Web

Analysis step: Remove empty droplets

Description

Find the empty droplets (double-negative droplets) in each well in a plate and assign these droplets to the EMPTY cluster.

See the README for more information about the algorithm used to find empty droplets.

Usage

remove_empty(plate)

Arguments

plate

A ddPCR plate.

Details

This function is recommended to be run as part of an analysis pipeline (ie. within the analyze function) rather than being called directly.

Value

A ddPCR plate with the empty droplets marked as empty. The plate's metadata will have a few new variables relating to the empty droplets.

Note

This is an S3 generic, which means that different ddPCR plate types can implement this function differently. See the README for more information on how to implement custom ddPCR plate types.

See Also

analyze
get_empty_cutoff

ddpcr documentation built on Aug. 21, 2023, 1:07 a.m.