Nothing
# Tests setup
set.seed(1)
library(palmerpenguins)
df <- as.data.frame(penguins[!apply(penguins, 1, function(x) { any(is.na(x)) }), ])
rownames(df) <- paste0("row", rownames(df))
# Dimensionality reduction
pca <- prcomp(df[, c("bill_length_mm", "bill_depth_mm", "flipper_length_mm", "body_mass_g")])
df <- cbind(df, pca$x)
de_df <- do.call(
rbind, lapply(
list(
c("Adelie", "Chinstrap"),
c("Adelie", "Gentoo"),
c("Chinstrap", "Gentoo")),
function(targs) {
this <- do.call(
rbind,
lapply(
c("bill_length_mm", "bill_depth_mm", "flipper_length_mm", "body_mass_g"),
function(obs) {
g1s <- as.vector(df$species==targs[1])
g2s <- as.vector(df$species==targs[2])
new <- data.frame(
feature = obs,
comparison = paste0(targs[1], "_vs_", targs[2]),
median_g1 = median(df[[obs]][g1s]),
median_g2 = median(df[[obs]][g2s]),
stringsAsFactors = FALSE
)
new$median_fold_change <- new$median_g1 / new$median_g2
new$median_log2_fold_change <- log2(new$median_fold_change)
new$p <- wilcox.test(x=df[[obs]][g1s],
y=df[[obs]][g2s])$p.value
new
})
)
this$`-log10(p)` <- -1* log10(this$p)
this$fdr <- p.adjust(this$p, method = "fdr")
this$`-log10(fdr)` <- -1* log10(this$fdr)
this
}
)
)
# Additional 'random' observations
df$groups <- sample(c("A","B","C","D","E"), nrow(df), replace = TRUE)
df$age <- sample(c("1","2","3","4"), nrow(df), replace = TRUE)
df$number <- as.numeric(seq_len(nrow(df)))
# For rows.use subsetting checks
rows.nums <- sort(sample(seq_len(nrow(df)), 40))
rows.names <- rownames(df)[rows.nums]
rows.logical <- seq_len(nrow(df)) %in% rows.nums
# Alternative colors
cols <- c("red", "blue", "yellow", "green", "black", "gray", "white")
# Remove the unneeded external data
rm(pca)
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