vinclozolin: Vinclozolin from AR in vitro assay
In drc: Analysis of Dose-Response Curves

Description Usage Format Details Source Examples

Dose-response experiment with vinclozolin in an AR reporter gene assay

1	data(vinclozolin)

A data frame with 53 observations on the following 3 variables.

exper: a factor with levels 10509 10821 10828 10904 11023 11106
conc: a numeric vector of concentrations of vinclozolin
effect: a numeric vector of luminescense effects

The basic dose-response experiment was repeated 6 times on different days. Chinese Hamster Ovary cells were exposed to various concentrations of vinclozolin for 22 hours and the resulting luminescense effects were recorded.

Data are part of mixture experiment reported in Nellemann et al (2003).

Nellemann C., Dalgaard M., Lam H.R. and Vinggaard A.M. (2003) The combined effects of vinclozolin and procymidone do not deviate from expected additivity in vitro and in vivo, Toxicological Sciences, 71, 251–262.

vinclozolin.m1 <- drm(effect~conc, exper, data=vinclozolin, fct = LL.3())
plot(vinclozolin.m1, xlim=c(0,50), ylim=c(0,2800), conLevel=1e-4)

vinclozolin.m2 <- drm(effect~conc, data=vinclozolin, fct = LL.3())
plot(vinclozolin.m2, xlim=c(0,50), conLevel=1e-4, add=TRUE, type="none", col="red")

## Are the ED50 values indetical across experiments?
vinclozolin.m3 <- update(vinclozolin.m1, pmodels=data.frame(exper, exper, 1))
anova(vinclozolin.m3, vinclozolin.m1)  # No!