drFitSpline: Function to fit smoothed splines to dose reponse data.

In grofit: The package was developed to fit fit many growth curves obtained under different conditions.

Description

The function fits dose response curves.

Usage

drFitSpline(conc, test, drID = "undefined",
            control = grofit.control())

Arguments

conc	Numeric vector, concentration (dose) data.
test	Numeric vector, response data beloning to conc.
drID	Character, identifying the dose response data.
control	Object of class grofit.control containing a list of options generated by the function grofit.control.

Details

The function uses the R internal function smooth.spline to fit a spline to the provided data. From the resulting curve the EC50 value is calculated.

Value

Generates an object of class drFit

raw.conc	Raw data provided to the function as conc.
raw.test	Raw data provided to the function as test.
drID	Character, identifying the dose response data.
fit.conc	Fitted concentration values.
fit.test	Fitted response values.
spline	nls object generated by the smooth.spline function.
fitFlag	Logical, indicating wether a spline could fitted successfully to data.
reliable	Logical, indicating wether the provided data is reliable (to be set manually).
control	Object of class grofit.control containing a list of options passed to the function as control.
parameters	List of parameters estimated from dose response curve fit.
EC50	Half maximal concentration.
yEC50	Response value related to EC50.
EC50.orig	EC50 value in original scale, if a transformation was applied.
xEC50.orig	Response value for EC50 in original scale, if a transformation was applied.

See Also

drFit, summary.drFitSpline, plot.drFitSpline

Examples

x <- 1:30
y <- 1/(1+exp(-0.5*(15-x)))+rnorm(30)/20
TestRun <- drFitSpline(x,y)
print(summary(TestRun))
plot(TestRun)

Example output

=== Dose response curve estimation ================
--- EC 50 -----------------------------------------
--> undefined
xEC50 14.8758758758759 yEC50 0.51559902180392


      EC50    yEC50 EC50.orig yEC50.orig
1 14.87588 0.515599  14.87588   0.515599

grofit documentation built on May 30, 2017, 4:08 a.m.