b_score: 2 way median polish

In platetools: Tools and Plots for Multi-Well Plates

2 way median polish

Description

2 way median polish to remove plate effects such as row/column/edge effects. Given a dataframe containing alpha-numeric wellIDs and numerical values, this b_score will return a dataframe of the same structure after a two-way median smooth.

Usage

b_score(data, well, plate, plate_id = NULL, normalise = FALSE)

Arguments

data	numeric data, either a vector or dataframe column
well	alpha-numeric wellIDs. e.g 'A01'
plate	numeric, number of wells within a plate
plate_id	Vector of plate_identifiers e.g "plate_01"
normalise	Boolean, whether or not to divide by ‘data'’s MAD

Examples

df <- data.frame(well = num_to_well(1:96),
                 vals = rnorm(96))

b_score(data = df$vals,
        well = df$well,
        plate = 96)

platetools documentation built on May 29, 2024, 8:43 a.m.