Description Usage Arguments Author(s) See Also
Designs primers for Amplicon Bisulfite sequencing experiments
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target |
|
genome |
default is NULL, it needs to be set only if
target is set to be a |
tag |
extra information about amplicons, this will
be concatanated to the names of BioStringSet object which
is either created internally or provided by the user by
supplying 'target' argument with a |
primer3 |
the filesystem path to primer3 executable, default value NULL will use the version installed with the package |
settings |
text file for p3 settings. Default value NULL will use the default settings file installed with the package. |
termoParam |
Location for thermodynamic parameters for primer3, this should designate a directory, not a single file. Default value NULL, will use the directory that is installed with the package. |
sizeRange |
a two element vector of integers for size range of the amplicons |
Tm |
a three element vector having minimum,optimum and maximum melting tempratures for primer desing (in that order). |
ncores |
number of cores to run on. default=1 |
Based on Arnaud Krebs' function, modified by Altuna Akalin
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