calculateVPrime: Calculate V'-Factor

In CeMOS-Mannheim/MALDIcellassay: Automated MALDI Cell Assays Using Dose-Response Curve Fitting

Calculate V'-Factor

Description

Calculate V'-Factor

Usage

calculateVPrime(res)

Arguments

res	Object of class MALDIassay

Details

The V'-factor is a generalization of the Z'-factor to a dose-response curve. See M.-A. Bray and A. Carpenter, Advanced assay development guidelines for image-based high content screening and analysis for details. It is defined as:

V' = 1 - 6 * \sigma_f/|\mu_p - \mu_n|

with

\sigma_f = \sqrt{1/N * \sum{y_fit - y_measured}^2}

In other words, \sigma_f is the standard deviation of residuals.

Note, we do not need to estimate the variance for the mean of the positive and negative value. So, this function uses the top and bottom asymptote directly instead of taking the top and bottom concentrations in consideration.

Value

Numeric vector of V'-factors

Examples

# see example for `fitCurve()` to see how this data was generated
data(Blank2022res)

calculateVPrime(Blank2022res) 

CeMOS-Mannheim/MALDIcellassay documentation built on Jan. 24, 2025, 11:17 p.m.