heatmap.3: Create heatmap
In ConesaLab/MDM: Complete MDM Network Analysis for 16S rRNA Data

Use heatmap.3 function to create heatmap with color legends for multiple columns and rows.

heatmap.3(x, Rowv = TRUE, Colv = if (symm) "Rowv" else TRUE, distfun = dist, hclustfun = hclust, dendrogram = c("both", "row", "column", "none"), symm = FALSE, scale = c("none", "row", "column"), na.rm = TRUE, revC = identical(Colv, "Rowv"), add.expr, breaks, symbreaks = max(x < 0, na.rm = TRUE) || scale != "none", col = "heat.colors", colsep, rowsep, sepcolor = "white", sepwidth = c(0.05, 0.05), cellnote, notecex = 1, notecol = "cyan", na.color = par("bg"), trace = c("none", "column", "row", "both"), tracecol = "cyan", hline = median(breaks), vline = median(breaks), linecol = tracecol, margins = c(5, 5), ColSideColors, RowSideColors, side.height.fraction = 0.3, cexRow = 0.2 + 1/log10(nr), cexCol = 0.2 + 1/log10(nc), labRow = NULL, labCol = NULL, key = TRUE, keysize = 1.5, density.info = c("none", "histogram", "density"), denscol = tracecol, symkey = max(x < 0, na.rm = TRUE) || symbreaks, densadj = 0.25, main = NULL, xlab = NULL, ylab = NULL, lmat = NULL, lhei = NULL, lwid = NULL, ColSideColorsSize = 1, RowSideColorsSize = 1, KeyValueName = "Value", ...)

`x`
`Rowv`
`Colv`
`distfun`
`hclustfun`
`dendrogram`
`symm`
`scale`
`na.rm`
`revC`
`add.expr`
`breaks`
`symbreaks`
`col`
`colsep`
`rowsep`
`sepcolor`
`sepwidth`
`cellnote`
`notecex`
`notecol`
`na.color`
`trace`
`tracecol`
`hline`
`vline`
`linecol`
`margins`
`ColSideColors`
`RowSideColors`
`side.height.fraction`
`cexRow`
`cexCol`
`labRow`
`labCol`
`key`
`keysize`
`density.info`
`denscol`
`symkey`
`densadj`
`main`
`xlab`
`ylab`
`lmat`
`lhei`
`lwid`
`ColSideColorsSize`
`RowSideColorsSize`
`KeyValueName`
`...`

##---- Should be DIRECTLY executable !! ----
##-- ==>  Define data, use random,
##--	or do  help(data=index)  for the standard data sets.

## The function is currently defined as
function (x, Rowv = TRUE, Colv = if (symm) "Rowv" else TRUE,
    distfun = dist, hclustfun = hclust, dendrogram = c("both",
        "row", "column", "none"), symm = FALSE, scale = c("none",
        "row", "column"), na.rm = TRUE, revC = identical(Colv,
        "Rowv"), add.expr, breaks, symbreaks = max(x < 0, na.rm = TRUE) ||
        scale != "none", col = "heat.colors", colsep, rowsep,
    sepcolor = "white", sepwidth = c(0.05, 0.05), cellnote, notecex = 1,
    notecol = "cyan", na.color = par("bg"), trace = c("none",
        "column", "row", "both"), tracecol = "cyan", hline = median(breaks),
    vline = median(breaks), linecol = tracecol, margins = c(5,
        5), ColSideColors, RowSideColors, side.height.fraction = 0.3,
    cexRow = 0.2 + 1/log10(nr), cexCol = 0.2 + 1/log10(nc), labRow = NULL,
    labCol = NULL, key = TRUE, keysize = 1.5, density.info = c("none",
        "histogram", "density"), denscol = tracecol, symkey = max(x <
        0, na.rm = TRUE) || symbreaks, densadj = 0.25, main = NULL,
    xlab = NULL, ylab = NULL, lmat = NULL, lhei = NULL, lwid = NULL,
    ColSideColorsSize = 1, RowSideColorsSize = 1, KeyValueName = "Value",
    ...)
{
    invalid <- function(x) {
        if (missing(x) || is.null(x) || length(x) == 0)
            return(TRUE)
        if (is.list(x))
            return(all(sapply(x, invalid)))
        else if (is.vector(x))
            return(all(is.na(x)))
        else return(FALSE)
    }
    x <- as.matrix(x)
    scale01 <- function(x, low = min(x), high = max(x)) {
        x <- (x - low)/(high - low)
        x
    }
    retval <- list()
    scale <- if (symm && missing(scale))
        "none"
    else match.arg(scale)
    dendrogram <- match.arg(dendrogram)
    trace <- match.arg(trace)
    density.info <- match.arg(density.info)
    if (length(col) == 1 && is.character(col))
        col <- get(col, mode = "function")
    if (!missing(breaks) && (scale != "none"))
        warning("Using scale=\"row\" or scale=\"column\" when breaks are",
            "specified can produce unpredictable results.", "Please consider using only one or the other.")
    if (is.null(Rowv) || is.na(Rowv))
        Rowv <- FALSE
    if (is.null(Colv) || is.na(Colv))
        Colv <- FALSE
    else if (Colv == "Rowv" && !isTRUE(Rowv))
        Colv <- FALSE
    if (length(di <- dim(x)) != 2 || !is.numeric(x))
        stop("`x' must be a numeric matrix")
    nr <- di[1]
    nc <- di[2]
    if (nr <= 1 || nc <= 1)
        stop("`x' must have at least 2 rows and 2 columns")
    if (!is.numeric(margins) || length(margins) != 2)
        stop("`margins' must be a numeric vector of length 2")
    if (missing(cellnote))
        cellnote <- matrix("", ncol = ncol(x), nrow = nrow(x))
    if (!inherits(Rowv, "dendrogram")) {
        if (((!isTRUE(Rowv)) || (is.null(Rowv))) && (dendrogram %in%
            c("both", "row"))) {
            if (is.logical(Colv) && (Colv))
                dendrogram <- "column"
            else dedrogram <- "none"
            warning("Discrepancy: Rowv is FALSE, while dendrogram is `",
                dendrogram, "'. Omitting row dendogram.")
        }
    }
    if (!inherits(Colv, "dendrogram")) {
        if (((!isTRUE(Colv)) || (is.null(Colv))) && (dendrogram %in%
            c("both", "column"))) {
            if (is.logical(Rowv) && (Rowv))
                dendrogram <- "row"
            else dendrogram <- "none"
            warning("Discrepancy: Colv is FALSE, while dendrogram is `",
                dendrogram, "'. Omitting column dendogram.")
        }
    }
    if (inherits(Rowv, "dendrogram")) {
        ddr <- Rowv
        rowInd <- order.dendrogram(ddr)
    }
    else if (is.integer(Rowv)) {
        hcr <- hclustfun(distfun(x))
        ddr <- as.dendrogram(hcr)
        ddr <- reorder(ddr, Rowv)
        rowInd <- order.dendrogram(ddr)
        if (nr != length(rowInd))
            stop("row dendrogram ordering gave index of wrong length")
    }
    else if (isTRUE(Rowv)) {
        Rowv <- rowMeans(x, na.rm = na.rm)
        hcr <- hclustfun(distfun(x))
        ddr <- as.dendrogram(hcr)
        ddr <- reorder(ddr, Rowv)
        rowInd <- order.dendrogram(ddr)
        if (nr != length(rowInd))
            stop("row dendrogram ordering gave index of wrong length")
    }
    else {
        rowInd <- nr:1
    }
    if (inherits(Colv, "dendrogram")) {
        ddc <- Colv
        colInd <- order.dendrogram(ddc)
    }
    else if (identical(Colv, "Rowv")) {
        if (nr != nc)
            stop("Colv = \"Rowv\" but nrow(x) != ncol(x)")
        if (exists("ddr")) {
            ddc <- ddr
            colInd <- order.dendrogram(ddc)
        }
        else colInd <- rowInd
    }
    else if (is.integer(Colv)) {
        hcc <- hclustfun(distfun(if (symm)
            x
        else t(x)))
        ddc <- as.dendrogram(hcc)
        ddc <- reorder(ddc, Colv)
        colInd <- order.dendrogram(ddc)
        if (nc != length(colInd))
            stop("column dendrogram ordering gave index of wrong length")
    }
    else if (isTRUE(Colv)) {
        Colv <- colMeans(x, na.rm = na.rm)
        hcc <- hclustfun(distfun(if (symm)
            x
        else t(x)))
        ddc <- as.dendrogram(hcc)
        ddc <- reorder(ddc, Colv)
        colInd <- order.dendrogram(ddc)
        if (nc != length(colInd))
            stop("column dendrogram ordering gave index of wrong length")
    }
    else {
        colInd <- 1:nc
    }
    retval$rowInd <- rowInd
    retval$colInd <- colInd
    retval$call <- match.call()
    x <- x[rowInd, colInd]
    x.unscaled <- x
    cellnote <- cellnote[rowInd, colInd]
    if (is.null(labRow))
        labRow <- if (is.null(rownames(x)))
            (1:nr)[rowInd]
        else rownames(x)
    else labRow <- labRow[rowInd]
    if (is.null(labCol))
        labCol <- if (is.null(colnames(x)))
            (1:nc)[colInd]
        else colnames(x)
    else labCol <- labCol[colInd]
    if (scale == "row") {
        retval$rowMeans <- rm <- rowMeans(x, na.rm = na.rm)
        x <- sweep(x, 1, rm)
        retval$rowSDs <- sx <- apply(x, 1, sd, na.rm = na.rm)
        x <- sweep(x, 1, sx, "/")
    }
    else if (scale == "column") {
        retval$colMeans <- rm <- colMeans(x, na.rm = na.rm)
        x <- sweep(x, 2, rm)
        retval$colSDs <- sx <- apply(x, 2, sd, na.rm = na.rm)
        x <- sweep(x, 2, sx, "/")
    }
    if (missing(breaks) || is.null(breaks) || length(breaks) <
        1) {
        if (missing(col) || is.function(col))
            breaks <- 16
        else breaks <- length(col) + 1
    }
    if (length(breaks) == 1) {
        if (!symbreaks)
            breaks <- seq(min(x, na.rm = na.rm), max(x, na.rm = na.rm),
                length = breaks)
        else {
            extreme <- max(abs(x), na.rm = TRUE)
            breaks <- seq(-extreme, extreme, length = breaks)
        }
    }
    nbr <- length(breaks)
    ncol <- length(breaks) - 1
    if (class(col) == "function")
        col <- col(ncol)
    min.breaks <- min(breaks)
    max.breaks <- max(breaks)
    x[x < min.breaks] <- min.breaks
    x[x > max.breaks] <- max.breaks
    if (missing(lhei) || is.null(lhei))
        lhei <- c(keysize, 4)
    if (missing(lwid) || is.null(lwid))
        lwid <- c(keysize, 4)
    if (missing(lmat) || is.null(lmat)) {
        lmat <- rbind(4:3, 2:1)
        if (!missing(ColSideColors)) {
            if (!is.character(ColSideColors) || nrow(ColSideColors) !=
                nc)
                stop("'ColSideColors' must be a matrix of nrow(x) rows")
            lmat <- rbind(lmat[1, ] + 1, c(NA, 1), lmat[2, ] +
                1)
            lhei = c(lhei[1], side.height.fraction * ColSideColorsSize/2,
                lhei[2])
        }
        if (!missing(RowSideColors)) {
            if (!is.character(RowSideColors) || ncol(RowSideColors) !=
                nr)
                stop("'RowSideColors' must be a matrix of ncol(x) columns")
            lmat <- cbind(lmat[, 1] + 1, c(rep(NA, nrow(lmat) -
                1), 1), lmat[, 2] + 1)
            lwid <- c(lwid[1], side.height.fraction * RowSideColorsSize/2,
                lwid[2])
        }
        lmat[is.na(lmat)] <- 0
    }
    if (length(lhei) != nrow(lmat))
        stop("lhei must have length = nrow(lmat) = ", nrow(lmat))
    if (length(lwid) != ncol(lmat))
        stop("lwid must have length = ncol(lmat) =", ncol(lmat))
    op <- par(no.readonly = TRUE)
    on.exit(par(op))
    layout(lmat, widths = lwid, heights = lhei, respect = FALSE)
    if (!missing(RowSideColors)) {
        if (!is.matrix(RowSideColors)) {
            par(mar = c(margins[1], 0, 0, 0.5))
            image(rbind(1:nr), col = RowSideColors[rowInd], axes = FALSE)
        }
        else {
            par(mar = c(margins[1], 0, 0, 0.5))
            rsc = t(RowSideColors[, rowInd, drop = F])
            rsc.colors = matrix()
            rsc.names = names(table(rsc))
            rsc.i = 1
            for (rsc.name in rsc.names) {
                rsc.colors[rsc.i] = rsc.name
                rsc[rsc == rsc.name] = rsc.i
                rsc.i = rsc.i + 1
            }
            rsc = matrix(as.numeric(rsc), nrow = dim(rsc)[1])
            image(t(rsc), col = as.vector(rsc.colors), axes = FALSE)
            if (length(rownames(RowSideColors)) > 0) {
                axis(1, 0:(dim(rsc)[2] - 1)/max(1, (dim(rsc)[2] -
                  1)), rownames(RowSideColors), las = 2, tick = FALSE)
            }
        }
    }
    if (!missing(ColSideColors)) {
        if (!is.matrix(ColSideColors)) {
            par(mar = c(0.5, 0, 0, margins[2]))
            image(cbind(1:nc), col = ColSideColors[colInd], axes = FALSE)
        }
        else {
            par(mar = c(0.5, 0, 0, margins[2]))
            csc = ColSideColors[colInd, , drop = F]
            csc.colors = matrix()
            csc.names = names(table(csc))
            csc.i = 1
            for (csc.name in csc.names) {
                csc.colors[csc.i] = csc.name
                csc[csc == csc.name] = csc.i
                csc.i = csc.i + 1
            }
            csc = matrix(as.numeric(csc), nrow = dim(csc)[1])
            image(csc, col = as.vector(csc.colors), axes = FALSE)
            if (length(colnames(ColSideColors)) > 0) {
                axis(2, 0:(dim(csc)[2] - 1)/max(1, (dim(csc)[2] -
                  1)), colnames(ColSideColors), las = 2, tick = FALSE)
            }
        }
    }
    par(mar = c(margins[1], 0, 0, margins[2]))
    x <- t(x)
    cellnote <- t(cellnote)
    if (revC) {
        iy <- nr:1
        if (exists("ddr"))
            ddr <- rev(ddr)
        x <- x[, iy]
        cellnote <- cellnote[, iy]
    }
    else iy <- 1:nr
    image(1:nc, 1:nr, x, xlim = 0.5 + c(0, nc), ylim = 0.5 +
        c(0, nr), axes = FALSE, xlab = "", ylab = "", col = col,
        breaks = breaks, ...)
    retval$carpet <- x
    if (exists("ddr"))
        retval$rowDendrogram <- ddr
    if (exists("ddc"))
        retval$colDendrogram <- ddc
    retval$breaks <- breaks
    retval$col <- col
    if (!invalid(na.color) & any(is.na(x))) {
        mmat <- ifelse(is.na(x), 1, NA)
        image(1:nc, 1:nr, mmat, axes = FALSE, xlab = "", ylab = "",
            col = na.color, add = TRUE)
    }
    axis(1, 1:nc, labels = labCol, las = 2, line = -0.5, tick = 0,
        cex.axis = cexCol)
    if (!is.null(xlab))
        mtext(xlab, side = 1, line = margins[1] - 1.25)
    axis(4, iy, labels = labRow, las = 2, line = -0.5, tick = 0,
        cex.axis = cexRow)
    if (!is.null(ylab))
        mtext(ylab, side = 4, line = margins[2] - 1.25)
    if (!missing(add.expr))
        eval(substitute(add.expr))
    if (!missing(colsep))
        for (csep in colsep) rect(xleft = csep + 0.5, ybottom = rep(0,
            length(csep)), xright = csep + 0.5 + sepwidth[1],
            ytop = rep(ncol(x) + 1, csep), lty = 1, lwd = 1,
            col = sepcolor, border = sepcolor)
    if (!missing(rowsep))
        for (rsep in rowsep) rect(xleft = 0, ybottom = (ncol(x) +
            1 - rsep) - 0.5, xright = nrow(x) + 1, ytop = (ncol(x) +
            1 - rsep) - 0.5 - sepwidth[2], lty = 1, lwd = 1,
            col = sepcolor, border = sepcolor)
    min.scale <- min(breaks)
    max.scale <- max(breaks)
    x.scaled <- scale01(t(x), min.scale, max.scale)
    if (trace %in% c("both", "column")) {
        retval$vline <- vline
        vline.vals <- scale01(vline, min.scale, max.scale)
        for (i in colInd) {
            if (!is.null(vline)) {
                abline(v = i - 0.5 + vline.vals, col = linecol,
                  lty = 2)
            }
            xv <- rep(i, nrow(x.scaled)) + x.scaled[, i] - 0.5
            xv <- c(xv[1], xv)
            yv <- 1:length(xv) - 0.5
            lines(x = xv, y = yv, lwd = 1, col = tracecol, type = "s")
        }
    }
    if (trace %in% c("both", "row")) {
        retval$hline <- hline
        hline.vals <- scale01(hline, min.scale, max.scale)
        for (i in rowInd) {
            if (!is.null(hline)) {
                abline(h = i + hline, col = linecol, lty = 2)
            }
            yv <- rep(i, ncol(x.scaled)) + x.scaled[i, ] - 0.5
            yv <- rev(c(yv[1], yv))
            xv <- length(yv):1 - 0.5
            lines(x = xv, y = yv, lwd = 1, col = tracecol, type = "s")
        }
    }
    if (!missing(cellnote))
        text(x = c(row(cellnote)), y = c(col(cellnote)), labels = c(cellnote),
            col = notecol, cex = notecex)
    par(mar = c(margins[1], 0, 0, 0))
    if (dendrogram %in% c("both", "row")) {
        plot(ddr, horiz = TRUE, axes = FALSE, yaxs = "i", leaflab = "none")
    }
    else plot.new()
    par(mar = c(0, 0, if (!is.null(main)) 5 else 0, margins[2]))
    if (dendrogram %in% c("both", "column")) {
        plot(ddc, axes = FALSE, xaxs = "i", leaflab = "none")
    }
    else plot.new()
    if (!is.null(main))
        title(main, cex.main = 1.5 * op[["cex.main"]])
    if (key) {
        par(mar = c(5, 4, 2, 1), cex = 0.75)
        tmpbreaks <- breaks
        if (symkey) {
            max.raw <- max(abs(c(x, breaks)), na.rm = TRUE)
            min.raw <- -max.raw
            tmpbreaks[1] <- -max(abs(x), na.rm = TRUE)
            tmpbreaks[length(tmpbreaks)] <- max(abs(x), na.rm = TRUE)
        }
        else {
            min.raw <- min(x, na.rm = TRUE)
            max.raw <- max(x, na.rm = TRUE)
        }
        z <- seq(min.raw, max.raw, length = length(col))
        image(z = matrix(z, ncol = 1), col = col, breaks = tmpbreaks,
            xaxt = "n", yaxt = "n")
        par(usr = c(0, 1, 0, 1))
        lv <- pretty(breaks)
        xv <- scale01(as.numeric(lv), min.raw, max.raw)
        axis(1, at = xv, labels = lv)
        if (scale == "row")
            mtext(side = 1, "Row Z-Score", line = 2)
        else if (scale == "column")
            mtext(side = 1, "Column Z-Score", line = 2)
        else mtext(side = 1, KeyValueName, line = 2)
        if (density.info == "density") {
            dens <- density(x, adjust = densadj, na.rm = TRUE)
            omit <- dens$x < min(breaks) | dens$x > max(breaks)
            dens$x <- dens$x[-omit]
            dens$y <- dens$y[-omit]
            dens$x <- scale01(dens$x, min.raw, max.raw)
            lines(dens$x, dens$y/max(dens$y) * 0.95, col = denscol,
                lwd = 1)
            axis(2, at = pretty(dens$y)/max(dens$y) * 0.95, pretty(dens$y))
            title("Color Key\nand Density Plot")
            par(cex = 0.5)
            mtext(side = 2, "Density", line = 2)
        }
        else if (density.info == "histogram") {
            h <- hist(x, plot = FALSE, breaks = breaks)
            hx <- scale01(breaks, min.raw, max.raw)
            hy <- c(h$counts, h$counts[length(h$counts)])
            lines(hx, hy/max(hy) * 0.95, lwd = 1, type = "s",
                col = denscol)
            axis(2, at = pretty(hy)/max(hy) * 0.95, pretty(hy))
            title("Color Key\nand Histogram")
            par(cex = 0.5)
            mtext(side = 2, "Count", line = 2)
        }
        else title("Color Key")
    }
    else plot.new()
    retval$colorTable <- data.frame(low = retval$breaks[-length(retval$breaks)],
        high = retval$breaks[-1], color = retval$col)
    invisible(retval)
  }