Description Usage Arguments Value Author(s) See Also Examples
View source: R/gating-functions.R
gate_interval_draw
constructs an interactive plotting window for user
to select the lower and upper bounds of a population (through mouse click)
which is constructed into a
rectangleGate
object and stored
in a filters
list. Both 1-D and 2-D
interval gates are supported, for 2-D interval gates an additional argument
axis
must be supplied to indicate which axis should be gated.
1 2 |
fr |
a |
alias |
the name(s) of the populations to be gated. If multiple
population names are supplied (e.g. |
channels |
vector of channel names to use for plotting, can be of length 1 for 1-D density histogram or length 2 for 2-D scatter plot. |
plot |
logical indicating whether the data should be plotted. This feature allows for constructing gates of different types over existing plots which may already contain a different gate type. |
axis |
indicates whether the |
label |
logical indicating whether to include
|
... |
additional arguments for |
afilters
list containing the
constructed rectangleGate
object(s).
Dillon Hammill (Dillon.Hammill@anu.edu.au)
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 | ## Not run:
# Copy and paste into console to interactively draw gates
library(CytoRSuiteData)
# Load in samples to flowSet
fs <- Activation
# Transform fluorescent channels
fs <- transform(fs, estimateLogicle(fs[[4]], cyto_fluor_channels(fs)))
# Get 1-D interval gate using gate_interval_draw - overlay control
ig <- gate_interval_draw(fs[[4]],
alias = "Cells",
channels = "PE-A",
overlay = fs[[1]],
density_stack = 0.5
)
# ig is a filters object - extract rectangleGate using `[[`
ig[[1]]
# Get 2-D interval gate on y axis using gate_interval_draw
ig <- gate_interval_draw(fs[[4]],
alias = "Cells",
channels = c("PE-A", "Alexa Fluor 488-A"),
axis = "y"
)
# ig is a filters object - extract rectangleGate using `[[`
ig[[1]]
## End(Not run)
|
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