HelenaLC/CATALYST: Cytometry dATa anALYSis Tools

Mass cytometry (CyTOF) uses heavy metal isotopes rather than fluorescent tags as reporters to label antibodies, thereby substantially decreasing spectral overlap and allowing for examination of over 50 parameters at the single cell level. While spectral overlap is significantly less pronounced in CyTOF than flow cytometry, spillover due to detection sensitivity, isotopic impurities, and oxide formation can impede data interpretability. We designed CATALYST (Cytometry dATa anALYSis Tools) to provide a pipeline for preprocessing of cytometry data, including i) normalization using bead standards, ii) single-cell deconvolution, and iii) bead-based compensation.

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Package details

Bioconductor views Clustering DataImport DifferentialExpression ExperimentalDesign FlowCytometry ImmunoOncology MassSpectrometry Normalization Preprocessing SingleCell Software StatisticalMethod Visualization
LicenseGPL (>=2)
URL https://github.com/HelenaLC/CATALYST
Package repositoryView on GitHub
Installation Install the latest version of this package by entering the following in R:
HelenaLC/CATALYST documentation built on Feb. 22, 2023, 12:04 p.m.