if (interactive()) savehistory();
library("aroma.affymetrix");
library("R.menu");
verbose <- Verbose(threshold=-10, timestamp=TRUE);
options(width=60);
chipType <- "MoGene-1_0-st-v1";
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# User settings
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
## setOption(aromaSettings, "user/initials", "HB");
## setOption(aromaSettings, "user/fullname", "Henrik Bengtsson");
## obf <- sprintf("%s@%s", "henrik.bengtsson", "aroma-project.org");
## setOption(aromaSettings, "user/email", obf);
## saveAnywhere(aromaSettings);
fullname <- getOption(aromaSettings, "user/fullname");
stopifnot(!is.null(fullname));
email <- getOption(aromaSettings, "user/email");
stopifnot(!is.null(email));
user <- getOption(aromaSettings, "user/initials");
stopifnot(!is.null(user));
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Settings
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
genomeVersions <- c("32"="mm8");
naVersions <- names(genomeVersions);
choices <- sprintf("na%s (%s)", naVersions, genomeVersions);
choice <- textMenu(choices, title="Choose NetAffx version: ", value=FALSE);
naVersion <- naVersions[choice];
genomeVersion <- genomeVersions[naVersion];
datestamp <- format(Sys.Date(), format="%Y%m%d");
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Setup required annotation files
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
cdf <- AffymetrixCdfFile$byChipType(chipType);
print(cdf);
ptb <- AffymetrixProbeTabFile$byChipType(chipType);
print(ptb);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Import UGP from PTB files
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
tags <- sprintf("na%s,%s,%s%s", naVersion, genomeVersion, user, datestamp);
tags <- sprintf("%s%s", user, datestamp);
tags <- c("build36", "mm8", tags);
ugp <- NULL;
tryCatch({
acp <- AromaCellPositionFile$byChipType(getChipType(cdf), tags=tags);
}, error = function(ex) {})
if (is.null(ugp)) {
acp <- AromaCellPositionFile$allocateFromCdf(cdf, tags=tags);
}
print(acp);
# Read unit names
colClasses <- c("*"="NULL", "probeID"="integer", "seqname"="character", "(start|stop)"="integer");
verbose && enter(verbose, "Read annotation data for all units");
data <- readDataFrame(ptb, colClasses=colClasses);
verbose && str(verbose, data);
verbose && exit(verbose);
verbose && enter(verbose, "Parse annotation data");
cells <- data$probeID;
cells <- Arguments$getIndices(cells, max=nbrOfCells(cdf));
chr <- data$seqname;
chr <- gsub("chr", "", chr, fixed=TRUE);
chr <- gsub("_random", "", chr, fixed=TRUE);
chr[chr == "X"] <- 23L;
chr[chr == "Y"] <- 24L;
chr[chr == "M"] <- 25L;
chr <- as.integer(chr);
position <- (data$start + data$stop) / 2;
verbose && print(verbose, table(chr));
verbose && exit(verbose);
verbose && enter(verbose, "Write to file");
acp[cells,1] <- chr;
acp[cells,2] <- position;
verbose && exit(verbose);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Update the file footer
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
srcFileTags <- list();
srcFiles <- c(list(cdf), list(ptb));
for (kk in seq_along(srcFiles)) {
srcFile <- srcFiles[[kk]];
tags <- list(
filename=getFilename(srcFile),
filesize=getFileSize(srcFile),
checksum=getChecksum(srcFile)
);
srcFileTags[[kk]] <- tags;
}
print(srcFileTags);
footer <- readFooter(acp);
footer$createdBy <- list(
fullname = fullname,
email = email
);
names(srcFileTags) <- sprintf("srcFile%d", seq_along(srcFileTags));
footer$srcFiles <- srcFileTags;
writeFooter(acp, footer);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Statistics
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
print(acp);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# WHAT'S NEW:
#
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
acp <- AromaUgpFile$byChipType("MoGene-1_0-st-v1,r3");
acp0 <- AromaAcpFile$byChipType("MoGene-1_0-st-v1,r3");
print(table(acp[,1], exclude=NULL));
print(table(acp0[,1], exclude=NULL));
## GenomeWideSNP_6,Full,na31,hg19,HB20110328
## 1 2 3 4 5 6 7 8
## 146626 153717 127767 120377 115735 112727 100925 98308
## 9 10 11 12 13 14 15 16
## 82300 93635 89598 87341 65927 57115 53596 54111
## 17 18 19 20 21 22 23 24
## 46609 52102 30365 43649 25105 24438 87271 9688
## 25 <NA>
## 455 1928
## GenomeWideSNP_6,na30,hg18,HB20100215.acp
## 1 2 3 4 5 6 7 8
## 146401 153663 127766 120296 115672 112825 100996 98277
## 9 10 11 12 13 14 15 16
## 82168 93592 89525 87321 66067 57103 53556 54182
## 17 18 19 20 21 22 23 24
## 46632 52093 30299 43628 25111 24484 87200 9483
## 25 <NA>
## 445 2630
rr <- whichVector(acp[,1] != acp0[,1]);
str(rr);
## integer(0)
rr <- whichVector(acp[,2] != acp0[,2]);
str(rr);
## integer(0)
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