asyr_grab96<-function(u){
require(dplyr)
pH<-c(rep(3.8,12),rep(5,12),rep(5.8,12),rep(6.6,12),rep(7.0,12),rep(7.4,12),rep(8.15,12),rep(9.2,12))
u %>%
.$LVL %>%
filter(.,Tick %in% tickfilter.A(Tick)) %>%
dplyr::select(.,counts=pHlvl,Tick,Well) %>%
dplyr::mutate(.,pH=pH[as.numeric(factor(Well))]) %>%
merge(.,data.frame(dye=c(rep('CL',6),rep('PR',6)),Well=unique(.['Well'])),by="Well") %>%
dplyr::group_by(.,Well,pH,dye) %>%
dplyr::summarise(.,counts=mean(counts)) %>%
dplyr::mutate(.,fl=u[['file']])
}
#####################
asyr_grabXFp<-function(u){
require(dplyr)
pH<-c(3.8,5,5.8,6.6,7.0,7.4,8.15,9.2)
u %>%
.$LVL %>%
dplyr::select(.,counts=pHlvl,Tick,Well)%>%
dplyr::filter(.,Tick %in% tickfilter.B(Tick)) %>%
dplyr::mutate(.,Tick=as.numeric(factor(Tick))) %>%
dplyr::mutate(.,dye = c("CL","PR")[as.numeric(factor(Tick>3))],pH=pH[as.numeric(factor(Well))]) %>%
dplyr::group_by(.,Well,pH,dye) %>%dplyr::summarise(.,counts=mean(counts)) %>%
dplyr::mutate(.,fl=u[['file']])
}
################################
asyr_grab24<-function(u){
require(dplyr)
pH=c(rep(3.8,3),rep(5,3),rep(5.8,3),rep(6.6,3),rep(7.0,3),rep(7.4,3),rep(8.15,3),rep(9.2,3))
u %>%
.$LVL %>%
dplyr::select(.,counts=pHlvl,Tick,Well)%>%
dplyr::filter(.,Tick %in% tickfilter.B(Tick)) %>%
dplyr::mutate(.,Tick=as.numeric(factor(Tick))) %>%
dplyr::mutate(.,dye = c("CL","PR")[as.numeric(factor(Tick>3))],pH=pH[as.numeric(factor(Well))]) %>%
dplyr::group_by(.,Well,pH,dye) %>%
dplyr::summarise(.,counts=mean(counts)) %>%
dplyr::mutate(.,fl=u[['file']])
}
##################
asyr_mungelist<-list(asyr_grab96,asyr_grab24,asyr_grabXFp)
####function to run pKa
###################
asyr_createRmd<-function (pHFluor, MFBatch, Directory) {
file.path(Directory,"data.feather") %>%
file.exists(.) %>%
if(.){DATA<<- "data.feather"}else{DATA<<- "data.csv"}
fp<-shQuote(file.path(Directory, DATA))
imp<-paste('rio::import(',fp,')')
readLines(system.file("rmd/pKaTemplate.Rmd", package = "PipeFish")) %>%
gsub("XBATCHX", MFBatch, .) %>%
gsub("XLOTX", pHFluor, .) %>%
gsub("rio::import('data.csv')",imp ,.)
}
intermediateStep<-function(u){
u %>%
XML::xmlTreeParse(.) %>%
PipeFish::Collect(.)
}
asyr_pKa<-function(pHFluor,MFBatch,Platform,Directory){
FileOut<-file.path(Directory,paste0(pHFluor,"pKa.Rmd"))
list.files(path=Directory,pattern='asyr',full.names = TRUE) %>%
lapply( .,PipeFish::intermediateStep) %>%
lapply(.,asyr_mungelist[[as.numeric(Platform)]]) %>%
dplyr::bind_rows() %>%
write.csv(x=.,file=file.path(Directory,"data.csv"),row.names=F)
asyr_createRmd(pHFluor,MFBatch,Directory) %>%
writeLines(text=.,con=file.path(Directory,paste0(pHFluor,"pKa.Rmd")),sep="\n")
rmarkdown::render(input=FileOut)
}
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