calc.extcoeff: Calculates extinction coefficients
In JPSieg/MeltR: Automated fitting of RNA/DNA absorbance melting curves and fluorescence binding isotherms in R
View source: R/Calc_exctinction_coef.R
calc.extcoeff R Documentation
Calculates extinction coefficients
Description
Calculates the extinction coefficient for a DNA or RNA sequences using the
nearest neighbor method. Also calculates the sum of all the extinction
coefficients in the input vector. Nearest neighbor parameters are from:
Usage
calc.extcoeff(NucAcid, wavelength = 260, silent = F)
Arguments
NucAcid
A vector containing RNA or DNA sequences. Example "c("RNA", "ACCUUUCG", "CGAAAGGU")"
wavelength
The wavelength the data was collected at
silent
To print the extinction coefficient once it is calculated
Details
Puglisi, J. D.; Tinoco, I. Absorbance Melting Curves of RNA.
Methods in Enzymology; RNA Processing Part A: General Methods;
Academic Press, 1989; Vol. 180, pp 304-325.
https://doi.org/10.1016/0076-6879(89)80108-9.
Value
A vector containing the extinction coefficient
JPSieg/MeltR documentation built on Feb. 4, 2024, 7:10 a.m.
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View source: R/Calc_exctinction_coef.R
| calc.extcoeff | R Documentation |
Calculates extinction coefficients
Description
Calculates the extinction coefficient for a DNA or RNA sequences using the nearest neighbor method. Also calculates the sum of all the extinction coefficients in the input vector. Nearest neighbor parameters are from:
Usage
calc.extcoeff(NucAcid, wavelength = 260, silent = F)
Arguments
NucAcid |
A vector containing RNA or DNA sequences. Example "c("RNA", "ACCUUUCG", "CGAAAGGU")" |
wavelength |
The wavelength the data was collected at |
silent |
To print the extinction coefficient once it is calculated |
Details
Puglisi, J. D.; Tinoco, I. Absorbance Melting Curves of RNA. Methods in Enzymology; RNA Processing Part A: General Methods; Academic Press, 1989; Vol. 180, pp 304-325. https://doi.org/10.1016/0076-6879(89)80108-9.
Value
A vector containing the extinction coefficient
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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