o3a.to.MeltR.csv: Pulls and combines data from a single wavelength from...

In JPSieg/MeltR: Automated fitting of RNA/DNA absorbance melting curves and fluorescence binding isotherms in R

Pulls and combines data from a single wavelength from multiple ".o3a" files

Description

Reads all ".o3a" files in a directory, pulls out the data from a single wavlength, and combines the data into a data frame for plotting and subsequent data analysis. Also includes an option to normalize the data to a reading and write the data frame to a ".csv" file.

Usage

o3a.to.MeltR.csv(
  file,
  write = FALSE,
  Wavelength = "260",
  Remove_readings = c(),
  Pathlength = rep(1, 1000),
  Sample_names = "NULL",
  Norm_reading = FALSE
)

Arguments

file	Path to a directory containing ".o3a" formatted files.
write	Optional prefix for the ".csv" formatted file you want to write. Example is "Name". Default = FALSE to not write a file.
Wavelength	Wavelength in nm that you want to pull out of the ".o3a" files. Default = "260".
Remove_readings	Optional reading to remove from the data set.
Pathlength	Pathlength in cm use to collect the data in the ".o3a" files, formatted into a vector. Example: Pathlength = c(1, 1, 0.5, 0.5, 0.1). Default = 1 cm.
Sample_names	Optional names of the samples in each ".o3a" file, Formatted into a vector. Example: Sample_names = c("Leo", "Theo", "Betty", "Apallo") or Sample_names = c(1:4). Default = NULL uses the ".o3a" file names as sample names
Norm_reading	Optional reading to normalize the data to. Example: Norm_reading = 1. Default = FALSE to not normalize the data.

Value

A data frame.

JPSieg/MeltR documentation built on Feb. 4, 2024, 7:10 a.m.