meltR.A.model: Models realistic absorbance melting curves

In JPSieg/MeltR: Automated fitting of RNA/DNA absorbance melting curves and fluorescence binding isotherms in R

Models realistic absorbance melting curves

Description

Models realistic absorbance melting curves

Usage

meltR.A.model(
  NucAcid = c("RNA", "CGCGCG"),
  Samples = 9,
  Absorbance_error = 0.01,
  Mmodel = "Homoduplex.2State",
  Tmodel = "VantHoff"
)

Arguments

NucAcid	A vector containing the Nucleic acid type and the sequences you are fitting for calculating extinction coefficients. Examples: c("RNA", "UUUUUU", "AAAAAA"), c("DNA", "GCTAGC"), etc... . For a custom extinction coefficient enter "Custom" followed by the molar extinction coefficients for every nucleic acid in the sample. For example, c("Custom", 10000, 20000).
Samples	The number of samples you want to model
Absorbance_error	The amount of absorbance error
Mmodel	The molecular model you want to fit. Options: "Monomolecular.2State", "Monomolecular.3State", "Heteroduplex.2State", "Homoduplex.2State".
Tmodel	The thermodynamic model you want to fit. Options: "VantHoff". Default = "VantHoff".

Value

A data frame containing modeled data

JPSieg/MeltR documentation built on Feb. 4, 2024, 7:10 a.m.