R/annoDomains.R
In PhanstielLab/plotgardener: Coordinate-Based Genomic Visualization Package for R
Defines functions
annoDomains
Documented in
annoDomains
#' Annotate domains in a Hi-C plot
#'
#' @usage annoDomains(
#' plot,
#' data,
#' half = "inherit",
#' linecolor = "black",
#' params = NULL,
#' ...
#' )
#'
#' @param plot Hi-C plot object from \code{plotHicSquare} or
#' \code{plotHicTriangle} on which to annotate pixels.
#' @param data A string specifying the BED file path, a dataframe in BED
#' format, or a \link[GenomicRanges]{GRanges} object specifying
#' domain ranges.
#' @param half Character value specifying which half of hic plots
#' to annotate. Triangle Hi-C plots will always default to the entirety of
#' the triangular plot. Default value is \code{half = "inherit"}. Options are:
#' \itemize{
#' \item{\code{"inherit"}: }{Domains will be annotated on the \code{half}
#' inherited by the input Hi-C plot.}
#' \item{\code{"both"}: }{Domains will be annotated on both halves of the
#' diagonal of a square Hi-C plot.}
#' \item{\code{"top"}: }{Domains will be annotated on the upper diagonal
#' half of a square Hi-C plot.}
#' \item{\code{"bottom"}: }{Domains will be annotated ont the bottom diagonal
#' half of a square Hi-C plot.}
#' }
#' @param linecolor A character value specifying the color of the domain
#' annotations. Default value is \code{linecolor = "black"}.
#' @param params An optional \link[plotgardener]{pgParams} object
#' containing relevant function parameters.
#' @param ... Additional grid graphical parameters. See \link[grid]{gpar}.
#'
#' @return Returns a \code{domain} object containing relevant
#' genomic region, placement, and \link[grid]{grob} information.
#'
#' @examples
#' ## Define a GRanges object with TAD ranges
#' library(GenomicRanges)
#' library(IRanges)
#' domains <- GRanges("chr21",
#' ranges = IRanges(
#' start = c(28210000, 29085000, 29430000, 29700000),
#' end = c(29085000, 29430000, 29700000, 30125000)
#' )
#' )
#'
#' ## Load Hi-C data
#' library(plotgardenerData)
#' data("IMR90_HiC_10kb")
#'
#' ## Create page
#' pageCreate(width = 4.5, height = 4, default.units = "inches")
#'
#' ## Plot and place a square Hi-C plot
#' hicPlot <- plotHicSquare(
#' data = IMR90_HiC_10kb, resolution = 10000,
#' zrange = c(0, 70),
#' chrom = "chr21",
#' chromstart = 28000000, chromend = 30300000,
#' assembly = "hg19",
#' x = 0.5, y = 0.5, width = 3, height = 3,
#' just = c("left", "top"),
#' default.units = "inches"
#' )
#'
#' ## Annotate domains on bottom half 0f Hi-C plot
#' annoDomains(
#' plot = hicPlot, data = domains,
#' half = "bottom", linecolor = "red"
#' )
#'
#' ## Annotate heatmap legend
#' annoHeatmapLegend(
#' plot = hicPlot,
#' x = 3.6, y = 0.5, width = 0.12, height = 1.2,
#' just = c("left", "top"), default.units = "inches"
#' )
#'
#' ## Annotate genome label
#' annoGenomeLabel(
#' plot = hicPlot, x = 0.5, y = 3.53, scale = "Mb",
#' just = c("left", "top")
#' )
#'
#' ## Hide page guides
#' pageGuideHide()
#' @export
annoDomains <- function(plot, data, half = "inherit",
linecolor = "black", params = NULL, ...) {
# =========================================================================
# FUNCTIONS
# =========================================================================
## Define a function that checks errors for annoDomains
errorcheck_annoDomains <- function(hic, half) {
###### hic #####
## check type of input for hic
if (!class(hic) %in% c(
"hicSquare", "hicTriangle",
"hicRectangle"
)) {
stop("Input plot must be a plot of class \'hicSquare\', ",
"\'hicTriangle\', or \'hicRectangle\'.",
call. = FALSE
)
}
###### half #####
## half needs to be a valid option
if (!half %in% c("inherit", "both", "top", "bottom")) {
stop("Invalid \'half\'. Options are \'inherit\', ",
"\'both\', \'top\', or \'bottom\'.",
call. = FALSE
)
}
## half needs to be able to align with what kind of hic plot is plotted
if (is(hic, "hicSquare")) {
if (hic$chrom == hic$altchrom) {
if ((hic$half == "top" | hic$half == "bottom") &&
(half == "both")) {
stop("Invalid \'half\' of plot to annotate.",
call. = FALSE
)
}
if (hic$half == "top" & half == "bottom") {
stop("Invalid \'half\' of plot to annotate.",
call. = FALSE
)
}
if (hic$half == "bottom" & half == "top") {
stop("Invalid \'half\' of plot to annotate.",
call. = FALSE
)
}
} else {
stop("Cannot annotate domains for an intrachromosomal ",
"square Hi-C plot.",
call. = FALSE
)
}
} else if (is(hic, "hicTriangle") |
is(hic, "hicRectangle")) {
if (half == "both" | half == "bottom") {
warning("Plot of class \'",
class(hic),
"\' detected. Pixels will automatically be annotated ",
"in the upper triangular of the plot.",
call. = FALSE
)
}
}
}
## Define a function that creates annotation grobs
domainAnnotation <- function(df, object, half) {
if (half == "bottom") {
x0_1 <- utils::type.convert(df["start"], as.is = TRUE)
x1_1 <- utils::type.convert(df["end"], as.is = TRUE)
y_1 <- utils::type.convert(df["start"], as.is = TRUE)
x_2 <- utils::type.convert(df["end"], as.is = TRUE)
y0_2 <- utils::type.convert(df["start"], as.is = TRUE)
y1_2 <- utils::type.convert(df["end"], as.is = TRUE)
seg1 <- segmentsGrob(
x0 = x0_1, x1 = x1_1,
y0 = y_1, y1 = y_1,
default.units = "native",
gp = object$gp
)
seg2 <- segmentsGrob(
x0 = x_2, x1 = x_2,
y0 = y0_2, y1 = y1_2,
default.units = "native",
gp = object$gp
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg1
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg2
),
envir = pgEnv
)
} else if (half == "top") {
x_1 <- utils::type.convert(df["start"], as.is = TRUE)
y0_1 <- utils::type.convert(df["start"], as.is = TRUE)
y1_1 <- utils::type.convert(df["end"], as.is = TRUE)
x0_2 <- utils::type.convert(df["start"], as.is = TRUE)
x1_2 <- utils::type.convert(df["end"], as.is = TRUE)
y_2 <- utils::type.convert(df["end"], as.is = TRUE)
seg1 <- segmentsGrob(
x0 = x_1, x1 = x_1,
y0 = y0_1, y1 = y1_1,
default.units = "native",
gp = object$gp
)
seg2 <- segmentsGrob(
x0 = x0_2, x1 = x1_2,
y0 = y_2, y1 = y_2,
default.units = "native",
gp = object$gp
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg1
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg2
),
envir = pgEnv
)
} else if (half == "both") {
x0_1 <- utils::type.convert(df["start"], as.is = TRUE)
x1_1 <- utils::type.convert(df["end"], as.is = TRUE)
y_1 <- utils::type.convert(df["start"], as.is = TRUE)
x_2 <- utils::type.convert(df["end"], as.is = TRUE)
y0_2 <- utils::type.convert(df["start"], as.is = TRUE)
y1_2 <- utils::type.convert(df["end"], as.is = TRUE)
x_1 <- utils::type.convert(df["start"], as.is = TRUE)
y0_1 <- utils::type.convert(df["start"], as.is = TRUE)
y1_1 <- utils::type.convert(df["end"], as.is = TRUE)
x0_2 <- utils::type.convert(df["start"], as.is = TRUE)
x1_2 <- utils::type.convert(df["end"], as.is = TRUE)
y_2 <- utils::type.convert(df["end"], as.is = TRUE)
seg1 <- segmentsGrob(
x0 = x0_1, x1 = x1_1,
y0 = y_1, y1 = y_1,
default.units = "native",
gp = object$gp
)
seg2 <- segmentsGrob(
x0 = x_2, x1 = x_2,
y0 = y0_2, y1 = y1_2,
default.units = "native",
gp = object$gp
)
seg3 <- segmentsGrob(
x0 = x_1, x1 = x_1,
y0 = y0_1, y1 = y1_1,
default.units = "native",
gp = object$gp
)
seg4 <- segmentsGrob(
x0 = x0_2, x1 = x1_2,
y0 = y_2, y1 = y_2,
default.units = "native",
gp = object$gp
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg1
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg2
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg3
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg4
),
envir = pgEnv
)
}
}
# =========================================================================
# PARSE PARAMETERS
# =========================================================================
domainsInternal <- parseParams(
params = params,
defaultArgs = formals(eval(
match.call()[[1]]
)),
declaredArgs = lapply(
match.call()[-1], eval.parent,
n = 2
),
class = "domainsInternal"
)
domainsInternal$gp <- setGP(
gpList = gpar(),
params = domainsInternal, ...
)
domainsInternal$gp$col <- domainsInternal$linecolor
domainsInternal$gp$lineend <- "square"
# =========================================================================
# INITIALIZE OBJECT: GET REGION/DIMENSIONS FROM HIC PLOT INPUT
# =========================================================================
domains <- structure(list(
hicClass = class(domainsInternal$plot),
chrom = domainsInternal$plot$chrom,
chromstart = domainsInternal$plot$chromstart,
chromend = domainsInternal$plot$chromend,
assembly = domainsInternal$plot$assembly,
x = domainsInternal$plot$x,
y = domainsInternal$plot$y,
width = domainsInternal$plot$width,
height = domainsInternal$plot$height,
just = domainsInternal$plot$just, grobs = NULL,
outsideVP = NULL
),
class = "domain"
)
# =========================================================================
# CATCH ERRORS
# =========================================================================
check_page(error = "Cannot annotate Hi-C domains without a
`plotgardener` page.")
if (is.null(domainsInternal$plot)) {
stop("argument \"plot\" is missing, ",
"with no default.",
call. = FALSE
)
}
if (is.null(domainsInternal$data)) {
stop("argument \"data\" is missing, ",
"with no default.",
call. = FALSE
)
}
errorcheck_annoDomains(
hic = domainsInternal$plot,
half = domainsInternal$half
)
# =========================================================================
# PARSE INHERITED HALF
# =========================================================================
half <- domainsInternal$half
if (half == "inherit") {
half <- inherit_half(hic = domainsInternal$plot)
}
if (is(domainsInternal$plot, "hicTriangle") |
is(domainsInternal$plot, "hicRectangle")) {
if (domainsInternal$plot$flip == TRUE){
half <- "bottom"
} else {
half <- "top"
}
}
# =========================================================================
# READ IN FILE, DATAFRAME OR GRANGES
# =========================================================================
bed <- read_rangeData(
data = domainsInternal$data,
assembly = domains$assembly
)
## chrom format and data chrom format
chromDataAgreement(data = bed, chrom = domains$chrom,
type = "ranges")
# =========================================================================
# SUBSET FOR DOMAINS IN REGION
# =========================================================================
bed_subset <- bed[which(bed[, "chrom"] == domains$chrom
& bed[, "start"] <=
domains$chromend & bed[, "end"] >=
domains$chromstart), ]
# =========================================================================
# VIEWPORTS
# =========================================================================
## Name viewport
currentViewports <- current_viewports()
vp_name <- paste0(
"domain",
length(grep(
pattern = "domain",
x = currentViewports
)) + 1
)
## Make viewport based on hic input viewport
if (is(domainsInternal$plot, "hicSquare")) {
vp <- viewport(
height = domainsInternal$plot$grobs$vp$height,
width = domainsInternal$plot$grobs$vp$width,
x = domainsInternal$plot$grobs$vp$x,
y = domainsInternal$plot$grobs$vp$y,
clip = "on",
xscale = domainsInternal$plot$grobs$vp$xscale,
yscale = domainsInternal$plot$grobs$vp$yscale,
just = domainsInternal$plot$grobs$vp$justification,
name = paste0(vp_name, "_inside")
)
vpClip <- NULL
} else if (is(domainsInternal$plot, "hicTriangle")) {
width <- convertUnit(domainsInternal$plot$outsideVP$width,
unitTo = get("page_units", pgEnv), valueOnly = TRUE
)
vp <- viewport(
height = unit(width / sqrt(2), get("page_units", pgEnv)),
width = unit(width / sqrt(2), get("page_units", pgEnv)),
x = unit(0, "npc"), y = unit(0, "npc"),
xscale = domainsInternal$plot$grobs$vp$xscale,
yscale = domainsInternal$plot$grobs$vp$yscale,
just = c("left", "bottom"),
name = paste0(vp_name, "_inside"),
angle = -45
)
vpClip <- viewport(
height = domainsInternal$plot$outsideVP$height,
width = domainsInternal$plot$outsideVP$width,
x = domainsInternal$plot$outsideVP$x,
y = domainsInternal$plot$outsideVP$y,
just = domainsInternal$plot$outsideVP$justification,
clip = "on",
name = paste0(vp_name, "_outside")
)
if (domainsInternal$plot$flip == TRUE){
vp$y <- unit(1, "npc")
}
} else if (is(domainsInternal$plot, "hicRectangle")) {
side <- convertUnit(domainsInternal$plot$grobs$vp$width,
unitTo = get("page_units", pgEnv)
)
vp <- viewport(
height = side, width = side,
x = unit(domainsInternal$plot$grobs$vp$xscale[1], "native"),
y = unit(0, "npc"),
xscale = domainsInternal$plot$grobs$vp$xscale,
yscale = domainsInternal$plot$grobs$vp$yscale,
just = c("left", "bottom"),
name = paste0(vp_name, "_inside"),
angle = -45
)
vpClip <- viewport(
height = domainsInternal$plot$outsideVP$height,
width = domainsInternal$plot$outsideVP$width,
x = domainsInternal$plot$outsideVP$x,
y = domainsInternal$plot$outsideVP$y,
just = domainsInternal$plot$outsideVP$justification,
xscale = domainsInternal$plot$outsideVP$xscale,
clip = "on",
name = paste0(vp_name, "_outside")
)
if (domainsInternal$plot$flip == TRUE){
vp$y <- unit(1, "npc")
}
}
# =========================================================================
# INITIALIZE GTREE
# =========================================================================
domains$outsideVP <- vpClip
assign("domain_grobs", gTree(vp = vp), envir = pgEnv)
# =========================================================================
# GROBS
# =========================================================================
if (nrow(bed_subset) > 0) {
invisible(apply(bed_subset, 1, domainAnnotation,
object = domainsInternal,
half = half
))
} else {
warning("No domains found in region.", call. = FALSE)
}
# =========================================================================
# ADD GROBS TO GLIST AND OBJECT
# =========================================================================
domains$grobs <- get("domain_grobs", envir = pgEnv)
if (!is.null(vpClip)) {
pushViewport(vpClip)
grid.draw(domains$grobs)
upViewport()
} else {
grid.draw(domains$grobs)
}
# =========================================================================
# RETURN OBJECT
# =========================================================================
message("domain[", vp_name, "]")
invisible(domains)
}
PhanstielLab/plotgardener documentation built on May 7, 2024, 4:21 a.m.
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Defines functions annoDomains
Documented in annoDomains
#' Annotate domains in a Hi-C plot
#'
#' @usage annoDomains(
#' plot,
#' data,
#' half = "inherit",
#' linecolor = "black",
#' params = NULL,
#' ...
#' )
#'
#' @param plot Hi-C plot object from \code{plotHicSquare} or
#' \code{plotHicTriangle} on which to annotate pixels.
#' @param data A string specifying the BED file path, a dataframe in BED
#' format, or a \link[GenomicRanges]{GRanges} object specifying
#' domain ranges.
#' @param half Character value specifying which half of hic plots
#' to annotate. Triangle Hi-C plots will always default to the entirety of
#' the triangular plot. Default value is \code{half = "inherit"}. Options are:
#' \itemize{
#' \item{\code{"inherit"}: }{Domains will be annotated on the \code{half}
#' inherited by the input Hi-C plot.}
#' \item{\code{"both"}: }{Domains will be annotated on both halves of the
#' diagonal of a square Hi-C plot.}
#' \item{\code{"top"}: }{Domains will be annotated on the upper diagonal
#' half of a square Hi-C plot.}
#' \item{\code{"bottom"}: }{Domains will be annotated ont the bottom diagonal
#' half of a square Hi-C plot.}
#' }
#' @param linecolor A character value specifying the color of the domain
#' annotations. Default value is \code{linecolor = "black"}.
#' @param params An optional \link[plotgardener]{pgParams} object
#' containing relevant function parameters.
#' @param ... Additional grid graphical parameters. See \link[grid]{gpar}.
#'
#' @return Returns a \code{domain} object containing relevant
#' genomic region, placement, and \link[grid]{grob} information.
#'
#' @examples
#' ## Define a GRanges object with TAD ranges
#' library(GenomicRanges)
#' library(IRanges)
#' domains <- GRanges("chr21",
#' ranges = IRanges(
#' start = c(28210000, 29085000, 29430000, 29700000),
#' end = c(29085000, 29430000, 29700000, 30125000)
#' )
#' )
#'
#' ## Load Hi-C data
#' library(plotgardenerData)
#' data("IMR90_HiC_10kb")
#'
#' ## Create page
#' pageCreate(width = 4.5, height = 4, default.units = "inches")
#'
#' ## Plot and place a square Hi-C plot
#' hicPlot <- plotHicSquare(
#' data = IMR90_HiC_10kb, resolution = 10000,
#' zrange = c(0, 70),
#' chrom = "chr21",
#' chromstart = 28000000, chromend = 30300000,
#' assembly = "hg19",
#' x = 0.5, y = 0.5, width = 3, height = 3,
#' just = c("left", "top"),
#' default.units = "inches"
#' )
#'
#' ## Annotate domains on bottom half 0f Hi-C plot
#' annoDomains(
#' plot = hicPlot, data = domains,
#' half = "bottom", linecolor = "red"
#' )
#'
#' ## Annotate heatmap legend
#' annoHeatmapLegend(
#' plot = hicPlot,
#' x = 3.6, y = 0.5, width = 0.12, height = 1.2,
#' just = c("left", "top"), default.units = "inches"
#' )
#'
#' ## Annotate genome label
#' annoGenomeLabel(
#' plot = hicPlot, x = 0.5, y = 3.53, scale = "Mb",
#' just = c("left", "top")
#' )
#'
#' ## Hide page guides
#' pageGuideHide()
#' @export
annoDomains <- function(plot, data, half = "inherit",
linecolor = "black", params = NULL, ...) {
# =========================================================================
# FUNCTIONS
# =========================================================================
## Define a function that checks errors for annoDomains
errorcheck_annoDomains <- function(hic, half) {
###### hic #####
## check type of input for hic
if (!class(hic) %in% c(
"hicSquare", "hicTriangle",
"hicRectangle"
)) {
stop("Input plot must be a plot of class \'hicSquare\', ",
"\'hicTriangle\', or \'hicRectangle\'.",
call. = FALSE
)
}
###### half #####
## half needs to be a valid option
if (!half %in% c("inherit", "both", "top", "bottom")) {
stop("Invalid \'half\'. Options are \'inherit\', ",
"\'both\', \'top\', or \'bottom\'.",
call. = FALSE
)
}
## half needs to be able to align with what kind of hic plot is plotted
if (is(hic, "hicSquare")) {
if (hic$chrom == hic$altchrom) {
if ((hic$half == "top" | hic$half == "bottom") &&
(half == "both")) {
stop("Invalid \'half\' of plot to annotate.",
call. = FALSE
)
}
if (hic$half == "top" & half == "bottom") {
stop("Invalid \'half\' of plot to annotate.",
call. = FALSE
)
}
if (hic$half == "bottom" & half == "top") {
stop("Invalid \'half\' of plot to annotate.",
call. = FALSE
)
}
} else {
stop("Cannot annotate domains for an intrachromosomal ",
"square Hi-C plot.",
call. = FALSE
)
}
} else if (is(hic, "hicTriangle") |
is(hic, "hicRectangle")) {
if (half == "both" | half == "bottom") {
warning("Plot of class \'",
class(hic),
"\' detected. Pixels will automatically be annotated ",
"in the upper triangular of the plot.",
call. = FALSE
)
}
}
}
## Define a function that creates annotation grobs
domainAnnotation <- function(df, object, half) {
if (half == "bottom") {
x0_1 <- utils::type.convert(df["start"], as.is = TRUE)
x1_1 <- utils::type.convert(df["end"], as.is = TRUE)
y_1 <- utils::type.convert(df["start"], as.is = TRUE)
x_2 <- utils::type.convert(df["end"], as.is = TRUE)
y0_2 <- utils::type.convert(df["start"], as.is = TRUE)
y1_2 <- utils::type.convert(df["end"], as.is = TRUE)
seg1 <- segmentsGrob(
x0 = x0_1, x1 = x1_1,
y0 = y_1, y1 = y_1,
default.units = "native",
gp = object$gp
)
seg2 <- segmentsGrob(
x0 = x_2, x1 = x_2,
y0 = y0_2, y1 = y1_2,
default.units = "native",
gp = object$gp
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg1
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg2
),
envir = pgEnv
)
} else if (half == "top") {
x_1 <- utils::type.convert(df["start"], as.is = TRUE)
y0_1 <- utils::type.convert(df["start"], as.is = TRUE)
y1_1 <- utils::type.convert(df["end"], as.is = TRUE)
x0_2 <- utils::type.convert(df["start"], as.is = TRUE)
x1_2 <- utils::type.convert(df["end"], as.is = TRUE)
y_2 <- utils::type.convert(df["end"], as.is = TRUE)
seg1 <- segmentsGrob(
x0 = x_1, x1 = x_1,
y0 = y0_1, y1 = y1_1,
default.units = "native",
gp = object$gp
)
seg2 <- segmentsGrob(
x0 = x0_2, x1 = x1_2,
y0 = y_2, y1 = y_2,
default.units = "native",
gp = object$gp
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg1
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg2
),
envir = pgEnv
)
} else if (half == "both") {
x0_1 <- utils::type.convert(df["start"], as.is = TRUE)
x1_1 <- utils::type.convert(df["end"], as.is = TRUE)
y_1 <- utils::type.convert(df["start"], as.is = TRUE)
x_2 <- utils::type.convert(df["end"], as.is = TRUE)
y0_2 <- utils::type.convert(df["start"], as.is = TRUE)
y1_2 <- utils::type.convert(df["end"], as.is = TRUE)
x_1 <- utils::type.convert(df["start"], as.is = TRUE)
y0_1 <- utils::type.convert(df["start"], as.is = TRUE)
y1_1 <- utils::type.convert(df["end"], as.is = TRUE)
x0_2 <- utils::type.convert(df["start"], as.is = TRUE)
x1_2 <- utils::type.convert(df["end"], as.is = TRUE)
y_2 <- utils::type.convert(df["end"], as.is = TRUE)
seg1 <- segmentsGrob(
x0 = x0_1, x1 = x1_1,
y0 = y_1, y1 = y_1,
default.units = "native",
gp = object$gp
)
seg2 <- segmentsGrob(
x0 = x_2, x1 = x_2,
y0 = y0_2, y1 = y1_2,
default.units = "native",
gp = object$gp
)
seg3 <- segmentsGrob(
x0 = x_1, x1 = x_1,
y0 = y0_1, y1 = y1_1,
default.units = "native",
gp = object$gp
)
seg4 <- segmentsGrob(
x0 = x0_2, x1 = x1_2,
y0 = y_2, y1 = y_2,
default.units = "native",
gp = object$gp
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg1
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg2
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg3
),
envir = pgEnv
)
assign("domain_grobs",
addGrob(
get("domain_grobs", envir = pgEnv),
child = seg4
),
envir = pgEnv
)
}
}
# =========================================================================
# PARSE PARAMETERS
# =========================================================================
domainsInternal <- parseParams(
params = params,
defaultArgs = formals(eval(
match.call()[[1]]
)),
declaredArgs = lapply(
match.call()[-1], eval.parent,
n = 2
),
class = "domainsInternal"
)
domainsInternal$gp <- setGP(
gpList = gpar(),
params = domainsInternal, ...
)
domainsInternal$gp$col <- domainsInternal$linecolor
domainsInternal$gp$lineend <- "square"
# =========================================================================
# INITIALIZE OBJECT: GET REGION/DIMENSIONS FROM HIC PLOT INPUT
# =========================================================================
domains <- structure(list(
hicClass = class(domainsInternal$plot),
chrom = domainsInternal$plot$chrom,
chromstart = domainsInternal$plot$chromstart,
chromend = domainsInternal$plot$chromend,
assembly = domainsInternal$plot$assembly,
x = domainsInternal$plot$x,
y = domainsInternal$plot$y,
width = domainsInternal$plot$width,
height = domainsInternal$plot$height,
just = domainsInternal$plot$just, grobs = NULL,
outsideVP = NULL
),
class = "domain"
)
# =========================================================================
# CATCH ERRORS
# =========================================================================
check_page(error = "Cannot annotate Hi-C domains without a
`plotgardener` page.")
if (is.null(domainsInternal$plot)) {
stop("argument \"plot\" is missing, ",
"with no default.",
call. = FALSE
)
}
if (is.null(domainsInternal$data)) {
stop("argument \"data\" is missing, ",
"with no default.",
call. = FALSE
)
}
errorcheck_annoDomains(
hic = domainsInternal$plot,
half = domainsInternal$half
)
# =========================================================================
# PARSE INHERITED HALF
# =========================================================================
half <- domainsInternal$half
if (half == "inherit") {
half <- inherit_half(hic = domainsInternal$plot)
}
if (is(domainsInternal$plot, "hicTriangle") |
is(domainsInternal$plot, "hicRectangle")) {
if (domainsInternal$plot$flip == TRUE){
half <- "bottom"
} else {
half <- "top"
}
}
# =========================================================================
# READ IN FILE, DATAFRAME OR GRANGES
# =========================================================================
bed <- read_rangeData(
data = domainsInternal$data,
assembly = domains$assembly
)
## chrom format and data chrom format
chromDataAgreement(data = bed, chrom = domains$chrom,
type = "ranges")
# =========================================================================
# SUBSET FOR DOMAINS IN REGION
# =========================================================================
bed_subset <- bed[which(bed[, "chrom"] == domains$chrom
& bed[, "start"] <=
domains$chromend & bed[, "end"] >=
domains$chromstart), ]
# =========================================================================
# VIEWPORTS
# =========================================================================
## Name viewport
currentViewports <- current_viewports()
vp_name <- paste0(
"domain",
length(grep(
pattern = "domain",
x = currentViewports
)) + 1
)
## Make viewport based on hic input viewport
if (is(domainsInternal$plot, "hicSquare")) {
vp <- viewport(
height = domainsInternal$plot$grobs$vp$height,
width = domainsInternal$plot$grobs$vp$width,
x = domainsInternal$plot$grobs$vp$x,
y = domainsInternal$plot$grobs$vp$y,
clip = "on",
xscale = domainsInternal$plot$grobs$vp$xscale,
yscale = domainsInternal$plot$grobs$vp$yscale,
just = domainsInternal$plot$grobs$vp$justification,
name = paste0(vp_name, "_inside")
)
vpClip <- NULL
} else if (is(domainsInternal$plot, "hicTriangle")) {
width <- convertUnit(domainsInternal$plot$outsideVP$width,
unitTo = get("page_units", pgEnv), valueOnly = TRUE
)
vp <- viewport(
height = unit(width / sqrt(2), get("page_units", pgEnv)),
width = unit(width / sqrt(2), get("page_units", pgEnv)),
x = unit(0, "npc"), y = unit(0, "npc"),
xscale = domainsInternal$plot$grobs$vp$xscale,
yscale = domainsInternal$plot$grobs$vp$yscale,
just = c("left", "bottom"),
name = paste0(vp_name, "_inside"),
angle = -45
)
vpClip <- viewport(
height = domainsInternal$plot$outsideVP$height,
width = domainsInternal$plot$outsideVP$width,
x = domainsInternal$plot$outsideVP$x,
y = domainsInternal$plot$outsideVP$y,
just = domainsInternal$plot$outsideVP$justification,
clip = "on",
name = paste0(vp_name, "_outside")
)
if (domainsInternal$plot$flip == TRUE){
vp$y <- unit(1, "npc")
}
} else if (is(domainsInternal$plot, "hicRectangle")) {
side <- convertUnit(domainsInternal$plot$grobs$vp$width,
unitTo = get("page_units", pgEnv)
)
vp <- viewport(
height = side, width = side,
x = unit(domainsInternal$plot$grobs$vp$xscale[1], "native"),
y = unit(0, "npc"),
xscale = domainsInternal$plot$grobs$vp$xscale,
yscale = domainsInternal$plot$grobs$vp$yscale,
just = c("left", "bottom"),
name = paste0(vp_name, "_inside"),
angle = -45
)
vpClip <- viewport(
height = domainsInternal$plot$outsideVP$height,
width = domainsInternal$plot$outsideVP$width,
x = domainsInternal$plot$outsideVP$x,
y = domainsInternal$plot$outsideVP$y,
just = domainsInternal$plot$outsideVP$justification,
xscale = domainsInternal$plot$outsideVP$xscale,
clip = "on",
name = paste0(vp_name, "_outside")
)
if (domainsInternal$plot$flip == TRUE){
vp$y <- unit(1, "npc")
}
}
# =========================================================================
# INITIALIZE GTREE
# =========================================================================
domains$outsideVP <- vpClip
assign("domain_grobs", gTree(vp = vp), envir = pgEnv)
# =========================================================================
# GROBS
# =========================================================================
if (nrow(bed_subset) > 0) {
invisible(apply(bed_subset, 1, domainAnnotation,
object = domainsInternal,
half = half
))
} else {
warning("No domains found in region.", call. = FALSE)
}
# =========================================================================
# ADD GROBS TO GLIST AND OBJECT
# =========================================================================
domains$grobs <- get("domain_grobs", envir = pgEnv)
if (!is.null(vpClip)) {
pushViewport(vpClip)
grid.draw(domains$grobs)
upViewport()
} else {
grid.draw(domains$grobs)
}
# =========================================================================
# RETURN OBJECT
# =========================================================================
message("domain[", vp_name, "]")
invisible(domains)
}
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