get_genome_fasta: Download genome (fasta), annotation (GTF) and contaminants

In Roleren/ORFik: Open Reading Frames in Genomics

Download genome (fasta), annotation (GTF) and contaminants

Description

This function automatically downloads (if files not already exists) genomes and contaminants specified for genome alignment. By default, it will use ensembl reference, upon completion, the function will store a file called file.path(output.dir, "outputs.rds") with the output paths of your completed genome/annotation downloads. For most non-model nonvertebrate organisms, you need my fork of biomartr for it to work: remotes::install_github("Roleren/biomartr) If you misspelled something or crashed, delete wrong files and run again.
Do remake = TRUE, to do it all over again.

Usage

get_genome_fasta(
  genome,
  output.dir,
  organism,
  assembly,
  assembly_type,
  db,
  gunzip
)

Arguments

genome	logical, default: TRUE, download genome of organism specified in "organism" argument. If FALSE, check if the downloaded file already exist. If you want to use a custom gtf from you hard drive, set GTF = FALSE, and assign: annotation <- getGenomeAndAnnotation(genome = FALSE) annotation["genome"] = "path/to/genome.fasta". Will download the primary assembly from Ensembl.
output.dir	directory to save downloaded data
organism	scientific name of organism, Homo sapiens, Danio rerio, Mus musculus, etc. See biomartr:::get.ensembl.info() for full list of supported organisms.
assembly	character, default is assembly = organism, which means getting the first assembly in list, otherwise the name of the assembly wanted, like "GCA_000005845" will get ecoli substrain k12, which is the most used ones for references. Usually ignore this for non bacterial species.
assembly_type	character, default c("primary_assembly", "toplevel"). Used for ensembl only, specifies the genome assembly type. Searches for both primary and toplevel, and if both are found, uses the first by order (so primary is prioritized by default). The Primary assembly should usually be used if it exists. The "primary assembly" contains all the top-level sequence regions, excluding alternative haplotypes and patches. If the primary assembly file is not present for a species (only defined for standard model organisms), that indicates that there were no haplotype/patch regions, and in such cases, the 'toplevel file is used. For more details see: ensembl tutorial
db	database to use for genome and GTF, default adviced: "ensembl" (remember to set assembly_type to "primary_assembly", else it will contain haplotypes, very large file!). Alternatives: "refseq" (reference assemblies) and "genbank" (all assemblies)
gunzip	logical, default TRUE, uncompress downloaded files that are zipped when downloaded, should be TRUE!

Details

Some files that are made after download:
- A fasta index for the genome
- A TxDb to speed up GTF/GFF reading
- Seperat of merged contaminant files
Files that can be made:
- Gene symbols (hgnc, etc)
- Uniprot ids (For name of protein structures)
If you want custom genome or gtf from you hard drive, assign existing paths like this:
annotation <- getGenomeAndAnnotation(GTF = "path/to/gtf.gtf", genome = "path/to/genome.fasta")

Value

a named character vector of path to genomes and gtf downloaded, and additional contaminants if used. If merge_contaminants is TRUE, will not give individual fasta files to contaminants, but only the merged one.

References

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919035/

See Also

Other STAR: STAR.align.folder(), STAR.align.single(), STAR.allsteps.multiQC(), STAR.index(), STAR.install(), STAR.multiQC(), STAR.remove.crashed.genome(), install.fastp()

Examples

## Get Saccharomyces cerevisiae genome and gtf (create txdb for R)
#getGenomeAndAnnotation("Saccharomyces cerevisiae", tempdir(), assembly_type = "toplevel")
## Download and add pseudo 5' UTRs
#getGenomeAndAnnotation("Saccharomyces cerevisiae", tempdir(), assembly_type = "toplevel",
#  pseudo_5UTRS_if_needed = 100)
## Get Danio rerio genome and gtf (create txdb for R)
#getGenomeAndAnnotation("Danio rerio", tempdir())

output.dir <- "/Bio_data/references/zebrafish"
## Get Danio rerio and Phix contamints to deplete during alignment
#getGenomeAndAnnotation("Danio rerio", output.dir, phix = TRUE)

## Optimize for ORFik (speed up for large annotations like human or zebrafish)
#getGenomeAndAnnotation("Danio rerio", tempdir(), optimize = TRUE)

# Drosophila melanogaster (toplevel exists only)
#getGenomeAndAnnotation("drosophila melanogaster", output.dir = file.path(config["ref"],
# "Drosophila_melanogaster_BDGP6"), assembly_type = "toplevel")
## How to save malformed refseq gffs:
## First run function and let it crash:
#annotation <- getGenomeAndAnnotation(organism = "Arabidopsis thaliana",
#  output.dir = "~/Desktop/test_plant/",
#  assembly_type = "primary_assembly", db = "refseq")
## Then apply a fix (example for linux, too long rows):
# fixed_gff <- fix_malformed_gff("~/Desktop/test_plant/Arabidopsis_thaliana_genomic_refseq.gff")
## Then updated arguments:
# annotation <- c(fixed_gff, "~/Desktop/test_plant/Arabidopsis_thaliana_genomic_refseq.fna")
# names(annotation) <- c("gtf", "genome")
# Then make the txdb (for faster R use)
# makeTxdbFromGenome(annotation["gtf"], annotation["genome"], organism = "Arabidopsis thaliana")

Roleren/ORFik documentation built on Dec. 18, 2024, 11:39 p.m.