MaxQtoMSstatsTMTFormat | R Documentation |
Generate MSstatsTMT required input format from MaxQuant output
MaxQtoMSstatsTMTFormat(
evidence,
proteinGroups,
annotation,
which.proteinid = "Proteins",
rmProt_Only.identified.by.site = FALSE,
useUniquePeptide = TRUE,
rmPSM_withfewMea_withinRun = TRUE,
rmProtein_with1Feature = FALSE,
summaryforMultipleRows = sum,
use_log_file = TRUE,
append = FALSE,
verbose = TRUE,
log_file_path = NULL,
...
)
evidence |
name of 'evidence.txt' data, which includes feature-level data. |
proteinGroups |
name of 'proteinGroups.txt' data. |
annotation |
data frame which contains column Run, Fraction, TechRepMixture, Mixture, Channel, BioReplicate, Condition. Refer to the example 'annotation.mq' for the meaning of each column. |
which.proteinid |
Use 'Proteins' (default) column for protein name. 'Leading.proteins' or 'Leading.razor.proteins' or 'Gene.names' can be used instead to get the protein ID with single protein. However, those can potentially have the shared peptides. |
rmProt_Only.identified.by.site |
TRUE will remove proteins with '+' in 'Only.identified.by.site' column from proteinGroups.txt, which was identified only by a modification site. FALSE is the default. |
useUniquePeptide |
TRUE(default) removes peptides that are assigned for more than one proteins. We assume to use unique peptide for each protein. |
rmPSM_withfewMea_withinRun |
TRUE (default) will remove the features that have 1 or 2 measurements within each Run. |
rmProtein_with1Feature |
TRUE will remove the proteins which have only 1 peptide and charge. Defaut is FALSE. |
summaryforMultipleRows |
sum(default) or max - when there are multiple measurements for certain feature in certain run, select the feature with the largest summation or maximal value. |
use_log_file |
logical. If TRUE, information about data processing will be saved to a file. |
append |
logical. If TRUE, information about data processing will be added to an existing log file. |
verbose |
logical. If TRUE, information about data processing wil be printed to the console. |
log_file_path |
character. Path to a file to which information about data processing will be saved. If not provided, such a file will be created automatically. If 'append = TRUE', has to be a valid path to a file. |
... |
additional parameters to 'data.table::fread'. |
data.frame of class "MSstatsTMT"
head(evidence)
head(proteinGroups)
head(annotation.mq)
input.mq <- MaxQtoMSstatsTMTFormat(evidence, proteinGroups, annotation.mq)
head(input.mq)
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