The pannot package provides tools to annotate and analyze annotation enrichment within sets of proteins or genes
Install the package from the github repository using:
devtools::install_github("VoisinneG/pannot")
library(pannot)
## Warning: replacing previous import 'IRanges::desc' by 'plyr::desc' when
## loading 'PSICQUIC'
Retrieve annotations from the enrichR database "GO_Biological_Process_2018" for a set of genes:
genes <- c("Itsn2","Eps15l1","Cbl","Cblb","Cltc1","Cd5","Cd6")
df <- get_annotations_enrichr(data = genes, dbs = "GO_Biological_Process_2018")
## Uploading data to Enrichr... Done.
## Querying GO_Biological_Process_2018... Done.
## Parsing results... Done.
print(df)
## names
## 1 Cbl
## 2 Cblb
## 3 Cd5
## 4 Cd6
## 5 Cltc1
## 6 Eps15l1
## 7 Itsn2
## GO_Biological_Process_2018
## 1 negative regulation of epidermal growth factor-activated receptor activity (GO:0007175);negative regulation of epidermal growth factor receptor signaling pathway (GO:0042059);negative regulation of protein tyrosine kinase activity (GO:0061099);regulation of epidermal growth factor-activated receptor activity (GO:0007176);epidermal growth factor receptor signaling pathway (GO:0007173);negative regulation of ERBB signaling pathway (GO:1901185);negative regulation of receptor activity (GO:2000272);entry of bacterium into host cell (GO:0035635);regulation of epidermal growth factor receptor signaling pathway (GO:0042058);ERBB signaling pathway (GO:0038127);positive regulation of epidermal growth factor receptor signaling pathway (GO:0045742);cellular response to interleukin-6 (GO:0071354);interleukin-6-mediated signaling pathway (GO:0070102);positive regulation of receptor-mediated endocytosis (GO:0048260);positive regulation of ERBB signaling pathway (GO:1901186);regulation of receptor-mediated endocytosis (GO:0048259);entry into host cell (GO:0030260);cellular response to transforming growth factor beta stimulus (GO:0071560);transmembrane receptor protein serine/threonine kinase signaling pathway (GO:0007178);cellular response to fibroblast growth factor stimulus (GO:0044344);positive regulation of phosphatidylinositol 3-kinase signaling (GO:0014068);transforming growth factor beta receptor signaling pathway (GO:0007179);fibroblast growth factor receptor signaling pathway (GO:0008543);positive regulation of endocytosis (GO:0045807);regulation of phosphatidylinositol 3-kinase signaling (GO:0014066);modification-dependent protein catabolic process (GO:0019941);transmembrane receptor protein tyrosine kinase signaling pathway (GO:0007169);positive regulation of intracellular signal transduction (GO:1902533);negative regulation of programmed cell death (GO:0043069);regulation of apoptotic process (GO:0042981);ubiquitin-dependent protein catabolic process (GO:0006511);protein modification by small protein conjugation (GO:0032446);cellular response to cytokine stimulus (GO:0071345);cytokine-mediated signaling pathway (GO:0019221);protein ubiquitination (GO:0016567);negative regulation of apoptotic process (GO:0043066)
## 2 negative regulation of epidermal growth factor-activated receptor activity (GO:0007175);negative regulation of epidermal growth factor receptor signaling pathway (GO:0042059);negative regulation of protein tyrosine kinase activity (GO:0061099);regulation of epidermal growth factor-activated receptor activity (GO:0007176);epidermal growth factor receptor signaling pathway (GO:0007173);negative regulation of receptor activity (GO:2000272);ERBB signaling pathway (GO:0038127);NLS-bearing protein import into nucleus (GO:0006607);protein import into nucleus (GO:0006606);proteolysis (GO:0006508)
## 3
## 4 immunological synapse formation (GO:0001771);lipopolysaccharide-mediated signaling pathway (GO:0031663);positive regulation of cytokine production involved in inflammatory response (GO:1900017);regulation of cytokine production involved in inflammatory response (GO:1900015);heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules (GO:0007157);acute inflammatory response (GO:0002526);regulation of T cell proliferation (GO:0042129);positive regulation of inflammatory response (GO:0050729);cellular response to lipopolysaccharide (GO:0071222);positive regulation of T cell proliferation (GO:0042102);positive regulation of lymphocyte proliferation (GO:0050671);positive regulation of cytokine production (GO:0001819);response to lipopolysaccharide (GO:0032496);positive regulation of T cell activation (GO:0050870);response to molecule of bacterial origin (GO:0002237);inflammatory response (GO:0006954);response to lipid (GO:0033993);cell-cell adhesion via plasma-membrane adhesion molecules (GO:0098742)
## 5
## 6 negative regulation of epidermal growth factor receptor signaling pathway (GO:0042059);negative regulation of ERBB signaling pathway (GO:1901185);regulation of epidermal growth factor receptor signaling pathway (GO:0042058)
## 7 positive regulation of dendrite extension (GO:1903861);regulation of dendrite extension (GO:1903859);positive regulation of developmental growth (GO:0048639);positive regulation of cell growth (GO:0030307);positive regulation of neuron projection development (GO:0010976)
Perform annotation enrichment analysis on a subset of the genes:
idx_subset = which(df$names %in% c("Eps15l1", "Cblb","Cbl"))
res <- annotation_enrichment_analysis(df, idx_subset = idx_subset, sep=";", showProgress = FALSE)
print(res)
## annot_terms
## 2 negative regulation of epidermal growth factor receptor signaling pathway _GO:0042059!
## 1 negative regulation of epidermal growth factor-activated receptor activity _GO:0007175!
## 3 negative regulation of protein tyrosine kinase activity _GO:0061099!
## 4 regulation of epidermal growth factor-activated receptor activity _GO:0007176!
## 5 epidermal growth factor receptor signaling pathway _GO:0007173!
## 6 negative regulation of ERBB signaling pathway _GO:1901185!
## 7 negative regulation of receptor activity _GO:2000272!
## 9 regulation of epidermal growth factor receptor signaling pathway _GO:0042058!
## 10 ERBB signaling pathway _GO:0038127!
## 8 entry of bacterium into host cell _GO:0035635!
## 11 positive regulation of epidermal growth factor receptor signaling pathway _GO:0045742!
## 12 cellular response to interleukin-6 _GO:0071354!
## 13 interleukin-6-mediated signaling pathway _GO:0070102!
## 14 positive regulation of receptor-mediated endocytosis _GO:0048260!
## 15 positive regulation of ERBB signaling pathway _GO:1901186!
## 16 regulation of receptor-mediated endocytosis _GO:0048259!
## 17 entry into host cell _GO:0030260!
## 18 cellular response to transforming growth factor beta stimulus _GO:0071560!
## 19 transmembrane receptor protein serine/threonine kinase signaling pathway _GO:0007178!
## 20 cellular response to fibroblast growth factor stimulus _GO:0044344!
## 21 positive regulation of phosphatidylinositol 3-kinase signaling _GO:0014068!
## 22 transforming growth factor beta receptor signaling pathway _GO:0007179!
## 23 fibroblast growth factor receptor signaling pathway _GO:0008543!
## 24 positive regulation of endocytosis _GO:0045807!
## 25 regulation of phosphatidylinositol 3-kinase signaling _GO:0014066!
## 26 modification-dependent protein catabolic process _GO:0019941!
## 27 transmembrane receptor protein tyrosine kinase signaling pathway _GO:0007169!
## 28 positive regulation of intracellular signal transduction _GO:1902533!
## 29 negative regulation of programmed cell death _GO:0043069!
## 30 regulation of apoptotic process _GO:0042981!
## 31 ubiquitin-dependent protein catabolic process _GO:0006511!
## 32 protein modification by small protein conjugation _GO:0032446!
## 33 cellular response to cytokine stimulus _GO:0071345!
## 34 cytokine-mediated signaling pathway _GO:0019221!
## 35 protein ubiquitination _GO:0016567!
## 36 negative regulation of apoptotic process _GO:0043066!
## 37 NLS-bearing protein import into nucleus _GO:0006607!
## 38 protein import into nucleus _GO:0006606!
## 39 proteolysis _GO:0006508!
## 41 immunological synapse formation _GO:0001771!
## 42 lipopolysaccharide-mediated signaling pathway _GO:0031663!
## 43 positive regulation of cytokine production involved in inflammatory response _GO:1900017!
## 44 regulation of cytokine production involved in inflammatory response _GO:1900015!
## 45 heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules _GO:0007157!
## 46 acute inflammatory response _GO:0002526!
## 47 regulation of T cell proliferation _GO:0042129!
## 48 positive regulation of inflammatory response _GO:0050729!
## 49 cellular response to lipopolysaccharide _GO:0071222!
## 50 positive regulation of T cell proliferation _GO:0042102!
## 51 positive regulation of lymphocyte proliferation _GO:0050671!
## 52 positive regulation of cytokine production _GO:0001819!
## 53 response to lipopolysaccharide _GO:0032496!
## 54 positive regulation of T cell activation _GO:0050870!
## 55 response to molecule of bacterial origin _GO:0002237!
## 56 inflammatory response _GO:0006954!
## 57 response to lipid _GO:0033993!
## 58 cell-cell adhesion via plasma-membrane adhesion molecules _GO:0098742!
## 59 positive regulation of dendrite extension _GO:1903861!
## 60 regulation of dendrite extension _GO:1903859!
## 61 positive regulation of developmental growth _GO:0048639!
## 62 positive regulation of cell growth _GO:0030307!
## 63 positive regulation of neuron projection development _GO:0010976!
## annot_type
## 2 GO_Biological_Process_2018
## 1 GO_Biological_Process_2018
## 3 GO_Biological_Process_2018
## 4 GO_Biological_Process_2018
## 5 GO_Biological_Process_2018
## 6 GO_Biological_Process_2018
## 7 GO_Biological_Process_2018
## 9 GO_Biological_Process_2018
## 10 GO_Biological_Process_2018
## 8 GO_Biological_Process_2018
## 11 GO_Biological_Process_2018
## 12 GO_Biological_Process_2018
## 13 GO_Biological_Process_2018
## 14 GO_Biological_Process_2018
## 15 GO_Biological_Process_2018
## 16 GO_Biological_Process_2018
## 17 GO_Biological_Process_2018
## 18 GO_Biological_Process_2018
## 19 GO_Biological_Process_2018
## 20 GO_Biological_Process_2018
## 21 GO_Biological_Process_2018
## 22 GO_Biological_Process_2018
## 23 GO_Biological_Process_2018
## 24 GO_Biological_Process_2018
## 25 GO_Biological_Process_2018
## 26 GO_Biological_Process_2018
## 27 GO_Biological_Process_2018
## 28 GO_Biological_Process_2018
## 29 GO_Biological_Process_2018
## 30 GO_Biological_Process_2018
## 31 GO_Biological_Process_2018
## 32 GO_Biological_Process_2018
## 33 GO_Biological_Process_2018
## 34 GO_Biological_Process_2018
## 35 GO_Biological_Process_2018
## 36 GO_Biological_Process_2018
## 37 GO_Biological_Process_2018
## 38 GO_Biological_Process_2018
## 39 GO_Biological_Process_2018
## 41 GO_Biological_Process_2018
## 42 GO_Biological_Process_2018
## 43 GO_Biological_Process_2018
## 44 GO_Biological_Process_2018
## 45 GO_Biological_Process_2018
## 46 GO_Biological_Process_2018
## 47 GO_Biological_Process_2018
## 48 GO_Biological_Process_2018
## 49 GO_Biological_Process_2018
## 50 GO_Biological_Process_2018
## 51 GO_Biological_Process_2018
## 52 GO_Biological_Process_2018
## 53 GO_Biological_Process_2018
## 54 GO_Biological_Process_2018
## 55 GO_Biological_Process_2018
## 56 GO_Biological_Process_2018
## 57 GO_Biological_Process_2018
## 58 GO_Biological_Process_2018
## 59 GO_Biological_Process_2018
## 60 GO_Biological_Process_2018
## 61 GO_Biological_Process_2018
## 62 GO_Biological_Process_2018
## 63 GO_Biological_Process_2018
## annot_names
## 2 negative regulation of epidermal growth factor receptor signaling pathway (GO:0042059)
## 1 negative regulation of epidermal growth factor-activated receptor activity (GO:0007175)
## 3 negative regulation of protein tyrosine kinase activity (GO:0061099)
## 4 regulation of epidermal growth factor-activated receptor activity (GO:0007176)
## 5 epidermal growth factor receptor signaling pathway (GO:0007173)
## 6 negative regulation of ERBB signaling pathway (GO:1901185)
## 7 negative regulation of receptor activity (GO:2000272)
## 9 regulation of epidermal growth factor receptor signaling pathway (GO:0042058)
## 10 ERBB signaling pathway (GO:0038127)
## 8 entry of bacterium into host cell (GO:0035635)
## 11 positive regulation of epidermal growth factor receptor signaling pathway (GO:0045742)
## 12 cellular response to interleukin-6 (GO:0071354)
## 13 interleukin-6-mediated signaling pathway (GO:0070102)
## 14 positive regulation of receptor-mediated endocytosis (GO:0048260)
## 15 positive regulation of ERBB signaling pathway (GO:1901186)
## 16 regulation of receptor-mediated endocytosis (GO:0048259)
## 17 entry into host cell (GO:0030260)
## 18 cellular response to transforming growth factor beta stimulus (GO:0071560)
## 19 transmembrane receptor protein serine/threonine kinase signaling pathway (GO:0007178)
## 20 cellular response to fibroblast growth factor stimulus (GO:0044344)
## 21 positive regulation of phosphatidylinositol 3-kinase signaling (GO:0014068)
## 22 transforming growth factor beta receptor signaling pathway (GO:0007179)
## 23 fibroblast growth factor receptor signaling pathway (GO:0008543)
## 24 positive regulation of endocytosis (GO:0045807)
## 25 regulation of phosphatidylinositol 3-kinase signaling (GO:0014066)
## 26 modification-dependent protein catabolic process (GO:0019941)
## 27 transmembrane receptor protein tyrosine kinase signaling pathway (GO:0007169)
## 28 positive regulation of intracellular signal transduction (GO:1902533)
## 29 negative regulation of programmed cell death (GO:0043069)
## 30 regulation of apoptotic process (GO:0042981)
## 31 ubiquitin-dependent protein catabolic process (GO:0006511)
## 32 protein modification by small protein conjugation (GO:0032446)
## 33 cellular response to cytokine stimulus (GO:0071345)
## 34 cytokine-mediated signaling pathway (GO:0019221)
## 35 protein ubiquitination (GO:0016567)
## 36 negative regulation of apoptotic process (GO:0043066)
## 37 NLS-bearing protein import into nucleus (GO:0006607)
## 38 protein import into nucleus (GO:0006606)
## 39 proteolysis (GO:0006508)
## 41 immunological synapse formation (GO:0001771)
## 42 lipopolysaccharide-mediated signaling pathway (GO:0031663)
## 43 positive regulation of cytokine production involved in inflammatory response (GO:1900017)
## 44 regulation of cytokine production involved in inflammatory response (GO:1900015)
## 45 heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules (GO:0007157)
## 46 acute inflammatory response (GO:0002526)
## 47 regulation of T cell proliferation (GO:0042129)
## 48 positive regulation of inflammatory response (GO:0050729)
## 49 cellular response to lipopolysaccharide (GO:0071222)
## 50 positive regulation of T cell proliferation (GO:0042102)
## 51 positive regulation of lymphocyte proliferation (GO:0050671)
## 52 positive regulation of cytokine production (GO:0001819)
## 53 response to lipopolysaccharide (GO:0032496)
## 54 positive regulation of T cell activation (GO:0050870)
## 55 response to molecule of bacterial origin (GO:0002237)
## 56 inflammatory response (GO:0006954)
## 57 response to lipid (GO:0033993)
## 58 cell-cell adhesion via plasma-membrane adhesion molecules (GO:0098742)
## 59 positive regulation of dendrite extension (GO:1903861)
## 60 regulation of dendrite extension (GO:1903859)
## 61 positive regulation of developmental growth (GO:0048639)
## 62 positive regulation of cell growth (GO:0030307)
## 63 positive regulation of neuron projection development (GO:0010976)
## N_annot freq_annot fold_change p_value p_value_adjust_fdr
## 2 3 1.0000000 2.333333 0.02857143 0.4285714
## 1 2 0.6666667 2.333333 0.14285714 0.4285714
## 3 2 0.6666667 2.333333 0.14285714 0.4285714
## 4 2 0.6666667 2.333333 0.14285714 0.4285714
## 5 2 0.6666667 2.333333 0.14285714 0.4285714
## 6 2 0.6666667 2.333333 0.14285714 0.4285714
## 7 2 0.6666667 2.333333 0.14285714 0.4285714
## 9 2 0.6666667 2.333333 0.14285714 0.4285714
## 10 2 0.6666667 2.333333 0.14285714 0.4285714
## 8 1 0.3333333 2.333333 0.42857143 0.4285714
## 11 1 0.3333333 2.333333 0.42857143 0.4285714
## 12 1 0.3333333 2.333333 0.42857143 0.4285714
## 13 1 0.3333333 2.333333 0.42857143 0.4285714
## 14 1 0.3333333 2.333333 0.42857143 0.4285714
## 15 1 0.3333333 2.333333 0.42857143 0.4285714
## 16 1 0.3333333 2.333333 0.42857143 0.4285714
## 17 1 0.3333333 2.333333 0.42857143 0.4285714
## 18 1 0.3333333 2.333333 0.42857143 0.4285714
## 19 1 0.3333333 2.333333 0.42857143 0.4285714
## 20 1 0.3333333 2.333333 0.42857143 0.4285714
## 21 1 0.3333333 2.333333 0.42857143 0.4285714
## 22 1 0.3333333 2.333333 0.42857143 0.4285714
## 23 1 0.3333333 2.333333 0.42857143 0.4285714
## 24 1 0.3333333 2.333333 0.42857143 0.4285714
## 25 1 0.3333333 2.333333 0.42857143 0.4285714
## 26 1 0.3333333 2.333333 0.42857143 0.4285714
## 27 1 0.3333333 2.333333 0.42857143 0.4285714
## 28 1 0.3333333 2.333333 0.42857143 0.4285714
## 29 1 0.3333333 2.333333 0.42857143 0.4285714
## 30 1 0.3333333 2.333333 0.42857143 0.4285714
## 31 1 0.3333333 2.333333 0.42857143 0.4285714
## 32 1 0.3333333 2.333333 0.42857143 0.4285714
## 33 1 0.3333333 2.333333 0.42857143 0.4285714
## 34 1 0.3333333 2.333333 0.42857143 0.4285714
## 35 1 0.3333333 2.333333 0.42857143 0.4285714
## 36 1 0.3333333 2.333333 0.42857143 0.4285714
## 37 1 0.3333333 2.333333 0.42857143 0.4285714
## 38 1 0.3333333 2.333333 0.42857143 0.4285714
## 39 1 0.3333333 2.333333 0.42857143 0.4285714
## 41 0 0.0000000 0.000000 1.00000000 1.0000000
## 42 0 0.0000000 0.000000 1.00000000 1.0000000
## 43 0 0.0000000 0.000000 1.00000000 1.0000000
## 44 0 0.0000000 0.000000 1.00000000 1.0000000
## 45 0 0.0000000 0.000000 1.00000000 1.0000000
## 46 0 0.0000000 0.000000 1.00000000 1.0000000
## 47 0 0.0000000 0.000000 1.00000000 1.0000000
## 48 0 0.0000000 0.000000 1.00000000 1.0000000
## 49 0 0.0000000 0.000000 1.00000000 1.0000000
## 50 0 0.0000000 0.000000 1.00000000 1.0000000
## 51 0 0.0000000 0.000000 1.00000000 1.0000000
## 52 0 0.0000000 0.000000 1.00000000 1.0000000
## 53 0 0.0000000 0.000000 1.00000000 1.0000000
## 54 0 0.0000000 0.000000 1.00000000 1.0000000
## 55 0 0.0000000 0.000000 1.00000000 1.0000000
## 56 0 0.0000000 0.000000 1.00000000 1.0000000
## 57 0 0.0000000 0.000000 1.00000000 1.0000000
## 58 0 0.0000000 0.000000 1.00000000 1.0000000
## 59 0 0.0000000 0.000000 1.00000000 1.0000000
## 60 0 0.0000000 0.000000 1.00000000 1.0000000
## 61 0 0.0000000 0.000000 1.00000000 1.0000000
## 62 0 0.0000000 0.000000 1.00000000 1.0000000
## 63 0 0.0000000 0.000000 1.00000000 1.0000000
## nodes_annot p_value_adjust_bonferroni N_annot_background
## 2 Cbl;Cblb;Eps15l1 1 3
## 1 Cbl;Cblb 1 2
## 3 Cbl;Cblb 1 2
## 4 Cbl;Cblb 1 2
## 5 Cbl;Cblb 1 2
## 6 Cbl;Eps15l1 1 2
## 7 Cbl;Cblb 1 2
## 9 Cbl;Eps15l1 1 2
## 10 Cbl;Cblb 1 2
## 8 Cbl 1 1
## 11 Cbl 1 1
## 12 Cbl 1 1
## 13 Cbl 1 1
## 14 Cbl 1 1
## 15 Cbl 1 1
## 16 Cbl 1 1
## 17 Cbl 1 1
## 18 Cbl 1 1
## 19 Cbl 1 1
## 20 Cbl 1 1
## 21 Cbl 1 1
## 22 Cbl 1 1
## 23 Cbl 1 1
## 24 Cbl 1 1
## 25 Cbl 1 1
## 26 Cbl 1 1
## 27 Cbl 1 1
## 28 Cbl 1 1
## 29 Cbl 1 1
## 30 Cbl 1 1
## 31 Cbl 1 1
## 32 Cbl 1 1
## 33 Cbl 1 1
## 34 Cbl 1 1
## 35 Cbl 1 1
## 36 Cbl 1 1
## 37 Cblb 1 1
## 38 Cblb 1 1
## 39 Cblb 1 1
## 41 1 1
## 42 1 1
## 43 1 1
## 44 1 1
## 45 1 1
## 46 1 1
## 47 1 1
## 48 1 1
## 49 1 1
## 50 1 1
## 51 1 1
## 52 1 1
## 53 1 1
## 54 1 1
## 55 1 1
## 56 1 1
## 57 1 1
## 58 1 1
## 59 1 1
## 60 1 1
## 61 1 1
## 62 1 1
## 63 1 1
## freq_annot_background nodes_annot_background
## 2 0.4285714 Cbl;Cblb;Eps15l1
## 1 0.2857143 Cbl;Cblb
## 3 0.2857143 Cbl;Cblb
## 4 0.2857143 Cbl;Cblb
## 5 0.2857143 Cbl;Cblb
## 6 0.2857143 Cbl;Eps15l1
## 7 0.2857143 Cbl;Cblb
## 9 0.2857143 Cbl;Eps15l1
## 10 0.2857143 Cbl;Cblb
## 8 0.1428571 Cbl
## 11 0.1428571 Cbl
## 12 0.1428571 Cbl
## 13 0.1428571 Cbl
## 14 0.1428571 Cbl
## 15 0.1428571 Cbl
## 16 0.1428571 Cbl
## 17 0.1428571 Cbl
## 18 0.1428571 Cbl
## 19 0.1428571 Cbl
## 20 0.1428571 Cbl
## 21 0.1428571 Cbl
## 22 0.1428571 Cbl
## 23 0.1428571 Cbl
## 24 0.1428571 Cbl
## 25 0.1428571 Cbl
## 26 0.1428571 Cbl
## 27 0.1428571 Cbl
## 28 0.1428571 Cbl
## 29 0.1428571 Cbl
## 30 0.1428571 Cbl
## 31 0.1428571 Cbl
## 32 0.1428571 Cbl
## 33 0.1428571 Cbl
## 34 0.1428571 Cbl
## 35 0.1428571 Cbl
## 36 0.1428571 Cbl
## 37 0.1428571 Cblb
## 38 0.1428571 Cblb
## 39 0.1428571 Cblb
## 41 0.1428571 Cd6
## 42 0.1428571 Cd6
## 43 0.1428571 Cd6
## 44 0.1428571 Cd6
## 45 0.1428571 Cd6
## 46 0.1428571 Cd6
## 47 0.1428571 Cd6
## 48 0.1428571 Cd6
## 49 0.1428571 Cd6
## 50 0.1428571 Cd6
## 51 0.1428571 Cd6
## 52 0.1428571 Cd6
## 53 0.1428571 Cd6
## 54 0.1428571 Cd6
## 55 0.1428571 Cd6
## 56 0.1428571 Cd6
## 57 0.1428571 Cd6
## 58 0.1428571 Cd6
## 59 0.1428571 Itsn2
## 60 0.1428571 Itsn2
## 61 0.1428571 Itsn2
## 62 0.1428571 Itsn2
## 63 0.1428571 Itsn2
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