iso_preprocess: Preprocess isoform-level count matrix for isoform switch.

In atienza-ipmc/isoswitch: What the Package Does (One Line, Title Case)

Preprocess isoform-level count matrix for isoform switch.

Description

Removes transcripts with low relative expression compared to the total gene expression. Filtering threshold is given as parameter. Single-isoform genes (after the filtering step) are also removed. The resulting matrix is stored as a new assay in the input Seurat object

Usage

iso_preprocess(obj, assay, new_assay, filter_threshold)

Arguments

obj	Input Seurat object
assay	Assay containing unfiltered isoform-level counts
new_assay	Name of the new assay to store pre-processed counts
filter_threshold	Filtering threshold expressed as percentage of total gene expression

Value

Input Seurat object with additional assay contaning pre-processed counts

atienza-ipmc/isoswitch documentation built on June 27, 2022, 12:22 p.m.