CreateSeuratObj: Wrapper around Seurat::CreateSeuratObject

In bimberlabinternal/OOSAP: OHSU-ONPRC Single-cell Analysis Package

Description

Create Seurat object from count data (usually from Read10X()). This also sets pct.mito.

Usage

CreateSeuratObj(
  seuratData = NA,
  project = NA,
  minFeatures = 25,
  minCells = 0,
  mitoGenesPattern = "^MT-",
  gtfFile = NA
)

Arguments

seuratData,	Seurat input data, usually from Read10X().
project,	Sets the project name for the Seurat object.
minFeatures,	Include cells where at least this many features are detected.
minCells,	Include features detected in at least this many cells.
mitoGenesPattern	The expression to use when identfying mitochondrial genes

Value

A Seurat object with p.mito calculated.

bimberlabinternal/OOSAP documentation built on Jan. 19, 2021, 2:47 a.m.