In calabrialab/ISAnalytics: Analyze gene therapy vector insertion sites data identified from genomics next generation sequencing reads for clonal tracking studies

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ISAnalytics

ISAnalytics is an R package developed to analyze gene therapy vector insertion sites data identified from genomics next generation sequencing reads for clonal tracking studies.

In gene therapy, stem cells are modified using viral vectors to deliver the therapeutic transgene and replace functional properties since the genetic modification is stable and inherited in all cell progeny. The retrieval and mapping of the sequences flanking the virus-host DNA junctions allows the identification of insertion sites (IS), essential for monitoring the evolution of genetically modified cells in vivo. A comprehensive toolkit for the analysis of IS is required to foster clonal tracking studies and supporting the assessment of safety and long term efficacy in vivo. This package is aimed at (1) supporting automation of IS workflow, (2) performing base and advance analysis for IS tracking (clonal abundance, clonal expansions and statistics for insertional mutagenesis, etc.), (3) providing basic biology insights of transduced stem cells in vivo.

The paper is available here https://academic.oup.com/bib/article/24/1/bbac551/6955274?login=false

Visit the package website

You can visit the package website to view documentation, vignettes and more.

• For the release version: ISAnalytics Website release
• For the devel version: ISAnalytics Website dev

NEWS

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Getting help

For help please contact the maintainer of the package or open an issue on GitHub.

calabrialab/ISAnalytics documentation built on Nov. 2, 2023, 8:57 p.m.