assembleCas9DualgRNALibraryOligo: Function to facilitate assembly of oligo pool synthesis for...
In chris-hsiung/bears01: What the Package Does (One Line, Title Case)
View source: R/assembleCas9DualgRNALibraryOligo.R
assembleCas9DualgRNALibraryOligo R Documentation
Function to facilitate assembly of oligo pool synthesis for Cas9 dual gRNA expression as in Replogle et al 2020
BsmBIstuffer defaults to sequence for containing BsmBI sitesß
REseq5p defaults to contain BstxI site
REseq3p defaults to contain BlpI site
Description
Function to facilitate assembly of oligo pool synthesis for Cas9 dual gRNA expression as in Replogle et al 2020
BsmBIstuffer defaults to sequence for containing BsmBI sitesß
REseq5p defaults to contain BstxI site
REseq3p defaults to contain BlpI site
Usage
assembleCas9DualgRNALibraryOligo(
spacer1,
spacer2,
BsmBIstuffer = "gtttcagagcgagacgtgcctgcaggatacgtctcagaaacatg",
REseq5p = "ccaccttgttg",
REseq3p = "gtttaagagctaagctg",
Adapter5p,
Adapter3p
)
Value
character containing assembled oligo sequence
chris-hsiung/bears01 documentation built on April 9, 2024, 2:01 a.m.
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View source: R/assembleCas9DualgRNALibraryOligo.R
| assembleCas9DualgRNALibraryOligo | R Documentation |
Function to facilitate assembly of oligo pool synthesis for Cas9 dual gRNA expression as in Replogle et al 2020 BsmBIstuffer defaults to sequence for containing BsmBI sitesß REseq5p defaults to contain BstxI site REseq3p defaults to contain BlpI site
Description
Function to facilitate assembly of oligo pool synthesis for Cas9 dual gRNA expression as in Replogle et al 2020 BsmBIstuffer defaults to sequence for containing BsmBI sitesß REseq5p defaults to contain BstxI site REseq3p defaults to contain BlpI site
Usage
assembleCas9DualgRNALibraryOligo(
spacer1,
spacer2,
BsmBIstuffer = "gtttcagagcgagacgtgcctgcaggatacgtctcagaaacatg",
REseq5p = "ccaccttgttg",
REseq3p = "gtttaagagctaagctg",
Adapter5p,
Adapter3p
)
Value
character containing assembled oligo sequence
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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