getBestCRISPickSpacers: Get Best CRISPick Spacers. As written is currently intended...

In chris-hsiung/bears01: What the Package Does (One Line, Title Case)

Get Best CRISPick Spacers. As written is currently intended for CRIPRko output from CRISPick.

Description

Get Best CRISPick Spacers. As written is currently intended for CRIPRko output from CRISPick.

Usage

getBestCRISPickSpacers(
  CRISPickfile,
  TTTVonly = TRUE,
  checkBsmbI = TRUE,
  removeTTTT = TRUE
)

Arguments

CRISPickfile	A data frame containing CRISPick results. Minimally must contain 'sgRNA Sequence' column, which must consist of DNA bases only
TTTVonly	logical indicates whether to keep only TTTV PAM spacers; defaults to TRUE
checkBsmbI	logical indicates whether to remove spacers containing BsmbI sites; defaults to TRUE
removeTTTT	logical indicates whether to remove spacers containing TTTT or start with TTT; defaults to TRUE

Value

After filtering out TTTT, TTTstart, BsmbI sites (optional), Offtarget matches to 'MAX', returns a data frame sorted by On-Target Efficacy Score.

Author(s)

Chris Hsiung

chris-hsiung/bears01 documentation built on April 9, 2024, 2:01 a.m.