context("Alignment")
test_that("align generates .fasta files", {
## We can't use the NCBI BSgenome name directly as the QuasR::qAlign genome
## parameter, because the NCBI metadata has NAs in the publication date
## which confuses QuasR. As a workaround, resave the genome as a file.
file_genome <- tempfile(pattern = "genome-", fileext = ".fasta")
on.exit(unlink(file_genome))
## Subset the genome using `head` to speed up the calculation from several
## minutes to tens of seconds.
xstringset <- as(as(bsgenome, "Views"), "XStringSet")
names(xstringset) <- names(bsgenome)
xstringset <- endoapply(xstringset, head, 100)
writeXStringSet(xstringset, file_genome)
## None of the `views` will map uniquely, so add in a uniquely mapping
## string to validate.
gr_unique <- GRanges("NC_008563:1-100", seqinfo = seqinfo(views))
views_1_unique <- Views(bsgenome, c(.gr, gr_unique))
## Rbowtie runs a subprocess, so one cannot suppress output in conventional
## ways.
result <- align(views_1_unique, file_genome)
expect_equal(result, gr_unique)
})
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