MergeMultipleSeuratObjects: Read multiple 10x run into Seurat objects and merge into a...

In crazyhottommy/scclusteval: Evaluate the single cell clustering

Description

Read multiple 10x run into Seurat objects and merge into a single Seurat object. The names of the list of paths will be prepended to the cell name.

Usage

MergeMultipleSeuratObjects(input_folders, do.normalize = FALSE, ...)

Arguments

input_folders	A named list of folder path for each run.
do.normalize	Whether or not normalize the data after mergeing, default is FALSE
...	Other parameters for CreatSeuratObject in the Seurat package

Value

A single merged Seurat object from mulitple 10x runs.

Examples

## Not run: 
library(fs)
library(here)
library(stringr)
input_folders<- dir_ls( path = here("data"), recursive = T) %>% path_dir() %>%
unique() %>% str_subset("mm10-1.2.0_premrna")
merged_seurat<- MergeMultipleSeuratObjects(input_folders)

## End(Not run)

crazyhottommy/scclusteval documentation built on Aug. 5, 2021, 3:20 p.m.