Man pages for ec363/fpcountr
Fluorescent Protein Calibration For Plate Readers
| aa_mass_data | Amino acid masses |
| calc_fpconc | Calculate FP concentration in molar units |
| calc_fppercell | Calculate FP per cell values |
| calibrate_flu | Convert arbitrary fluorescence units to calibrated units |
| calibrate_od | Convert arbitrary optical density units to calibrated units |
| cell_quench_data | cell quench data |
| correct_flu | Correct normalised fluorescence to compensate for cellular... |
| find_columns | Find columns of a given plate type |
| find_plate_format | Find row and column configuration of a given plate type |
| find_rows | Find rows of a given plate type |
| find_wells | Find wells of a given plate format |
| find_wells_in_column | List of all wells in a specific column of a given plate type |
| find_wells_in_row | List of all wells in a specific row of a given plate type |
| find_wells_in_rowcolumn | List of all wells in specific rows and columns of a given... |
| flu_norm | Normalise fluorescence against negative well autofluorescence |
| fpbase_data | fluorescent protein properties from fpbase |
| generate_cfs | Generate conversion factors for FP calibrations |
| get_conc_A280 | Get FP concentrations using A280 method |
| get_conc_bca | Get FP concentrations using bicinchoninic acid (BCA) assay... |
| get_conc_ECmax | Get FP concentrations using ECmax method |
| get_extcoeff_A280 | Get a protein's molar extinction coefficient at A280 |
| get_fpbase_properties | Get properties of an FP from FPbase |
| get_kfactor | Get k-factor of a buffer |
| get_mw | Get molecular weight of a protein |
| get_pathlength | Get path length in a typical 96-well plate |
| kfactors_buffers_data | k-factors of different buffers |
| kfactors_temperature_data | k-factors of water at different temperatures |
| od_norm | Normalise optical density against blank wells |
| parse_magellan | Parse Tecan Spark/Magellan data from endpoint and kinetic... |
| parse_magellan_spectrum | Parse Tecan Spark/Magellan data from absorbance spectrum data |
| pathlength_water_data | pathlength vs volume data |
| process_absorbance_spectrum | Process absorbance spectrum data |
| process_plate | Process experimental data (normalise, correct and calibrate) |
| protein_seq_data | protein sequence data |
| save_metadata_template | Save a template metadata file |
| view_kfactors | View k-factors |
