calibrate_flu: Convert arbitrary fluorescence units to calibrated units

In ec363/fpcountr: Fluorescent Protein Calibration For Plate Readers

Convert arbitrary fluorescence units to calibrated units

Description

Used by process_plate function for fluorescence calibration. Function adds calibrated fluorescence column to the data, which is returned. Originally based on flopr::calibrate_flu, but with multiple changes. A list of arguments have been added to allow selection of required conversion factor from table that may include conversion factors from multiple instruments, FPs, etc., and function now includes error checks that report to the user if conversion factors are missing.

Usage

calibrate_flu(
  pr_data,
  flu_instr,
  flu_channel,
  flu_gain,
  flu_slug,
  flu_label,
  do_quench_correction = do_quench_correction,
  conversion_factors_csv
)

Arguments

pr_data	a data.frame of parsed plate reader data
flu_instr	instrument name
flu_channel	fluorescent channel name
flu_gain	gain
flu_slug	name of fluorescent protein in FPbase slug format
conversion_factors_csv	path of the CSV file containing predicted conversion factors for the fluorescent channels

Value

an updated data.frame with an additional column for calibrated fluorescence

ec363/fpcountr documentation built on Nov. 29, 2024, 12:03 p.m.