correct_flu: Correct normalised fluorescence to compensate for cellular...
In ec363/fpcountr: Fluorescent Protein Calibration For Plate Readers
correct_flu R Documentation
Correct normalised fluorescence to compensate for cellular quenching effects
Description
Used by process_plate function for cell quench correction. The presence of
cells, particularly at high densities, can 'quench' fluorescence such that
fluorescence readings are lower than expected from the equivalent FP
concentration in vitro. This function compensates for this effect using
empirical data that relates the fold decrease in fluorescence expected, given
a certain cell density.
Usage
correct_flu(pr_data, od_type, flu_channel)
Arguments
pr_data
a data.frame of parsed plate reader data
od_type
Which OD-type was used? Required for quench correction.
"OD600" or "OD700".
flu_channel
fluorescent channel name
Value
an updated data.frame with an additional column for corrected fluorescence
ec363/fpcountr documentation built on Nov. 29, 2024, 12:03 p.m.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
| correct_flu | R Documentation |
Correct normalised fluorescence to compensate for cellular quenching effects
Description
Used by process_plate function for cell quench correction. The presence of
cells, particularly at high densities, can 'quench' fluorescence such that
fluorescence readings are lower than expected from the equivalent FP
concentration in vitro. This function compensates for this effect using
empirical data that relates the fold decrease in fluorescence expected, given
a certain cell density.
Usage
correct_flu(pr_data, od_type, flu_channel)
Arguments
pr_data |
a data.frame of parsed plate reader data |
od_type |
Which OD-type was used? Required for quench correction. "OD600" or "OD700". |
flu_channel |
fluorescent channel name |
Value
an updated data.frame with an additional column for corrected fluorescence
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
Embedding an R snippet on your website
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.