get_kfactor: Get k-factor of a buffer

In ec363/fpcountr: Fluorescent Protein Calibration For Plate Readers

Get k-factor of a buffer

Description

Calculates k-factor of a given buffer/temperature combination according to data from Thermo Scientific for different buffers and temperatures. The k-factor is defined as the A975-A900 for a 1cm pathlength. The calculations are made under the assumption that the fold changes for buffers from water, and for temperatures from 25oC may be combined to get an approximate estimate of the k-factor for a given condition.

Usage

get_kfactor(
  buffer_used = "water",
  concentration_used = 0,
  temperature_used = 25
)

Arguments

buffer_used	string corresponding to buffer. Must match a buffer entry in dataset kfactors_buffers_data. Default is "water".
concentration_used	numeric value of concentration of buffer used. Default is 0 (pure water), so it needs changing if buffer isn't water. Take care to ensure the units are correct (buffers specified in M require concentrations in M not mM). This value is ignored if buffer = "water".
temperature_used	numeric value of temperature in oC. Default is 25.

Examples

get_kfactor(buffer_used = "TBS", concentration_used = 0.005, temperature_used = 30)

ec363/fpcountr documentation built on Nov. 29, 2024, 12:03 p.m.